ECH1 Double Nickase Plasmid (h): sc-406076-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ECH1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ECH1 Double Nickase Plasmid (h) and ECH1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ECH1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ECH1 Antibody (B-3): sc-515270
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ECH1 Double Nickase Plasmid (h) | sc-406076-NIC | 20 µg | $410.00 | |||
ECH1 Double Nickase Plasmid (h2) | sc-406076-NIC-2 | 20 µg | $410.00 |
ECH1 (enoyl-CoA hydratase 1) is a mitochondrial enzyme that catalyzes hydration steps in fatty-acid β-oxidation and participates in peroxisomal metabolism of unsaturated and branched-chain acyl-CoAs. By regulating acyl-CoA flux and downstream acetyl-CoA production, ECH1 links lipid catabolism to cellular bioenergetics, redox balance, and metabolic stress responses. Altered ECH1 expression or activity has been associated with metabolic reprogramming in disorders involving mitochondrial function and lipid handling, and it is frequently studied in the context of oxidative stress and energy-demanding tissues. These features make ECH1 a useful target for investigating mitochondrial fatty-acid oxidation pathways and their crosstalk with broader metabolic networks.
ECH1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ECH1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ECH1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ECH1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ECH1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.