Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DUOX1 CRISPR/Cas9 KO Plasmid (h): sc-402745
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DUOX1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DUOX1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DUOX1 Antibody (H-9): sc-393096
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DUOX1 CRISPR/Cas9 KO Plasmid (h) | sc-402745 | 20 µg | $397.00 |
Overview
DUOX1 (dual oxidase 1) encodes a membrane-associated NADPH oxidase that generates hydrogen peroxide at the cell surface, contributing to redox signaling and host defense at mucosal and epithelial barriers. Its peroxidase-like domain and EF-hand calcium-binding motifs support regulated ROS production that influences innate immune responses, epithelial differentiation, and wound repair programs. DUOX1-derived oxidants modulate signaling nodes such as MAPK and NF-κB pathways and can affect cytokine and chemokine expression in airway and gastrointestinal tissues. Dysregulated DUOX1 activity and altered oxidative microenvironments have been associated with inflammatory airway disease biology and cancer-relevant changes including epigenetic silencing and shifts in oxidative stress responses.
DUOX1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DUOX1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DUOX1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DUOX1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DUOX1 protein expression.
This CRISPR knockout system enables efficient generation of DUOX1-deficient cell models for investigation of DUOX1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DUOX1 exon(s) critical for DUOX1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DUOX1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DUOX1 CRISPR/Cas9 KO Plasmid (h) and DUOX1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DUOX1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DUOX1 HDR Plasmid (h) and DUOX1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DUOX1 homology arms to support homology-directed repair at defined DUOX1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.