DPPX Double Nickase Plasmid (h): sc-403662-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DPPX Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DPPX Double Nickase Plasmid (h) and DPPX Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DPP6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DPPX Antibody (A-8): sc-365147
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DPPX Double Nickase Plasmid (h) | sc-403662-NIC | 20 µg | $410.00 | |||
DPPX Double Nickase Plasmid (h2) | sc-403662-NIC-2 | 20 µg | $410.00 |
DPP6 encodes dipeptidyl peptidase-like protein 6 (DPPX), an enzymatically inactive member of the DPP family that functions as an auxiliary subunit of voltage-gated potassium channels, particularly Kv4 complexes. By modulating channel gating, trafficking, and surface expression, DPPX shapes dendritic excitability, action potential repolarization, and synaptic integration in neurons, linking it to activity-dependent signaling and neural circuit homeostasis. Altered DPP6/DPPX expression or genetic variation has been associated with neurodevelopmental and neuropsychiatric phenotypes as well as susceptibility to neuronal hyperexcitability-related disorders, making it relevant for studies of ion channel regulation and brain function.
DPPX Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DPP6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DPP6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DPP6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DPP6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.