DMRT1 CRISPR/Cas9 KO Plasmid (m): sc-424507
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DMRT1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DMRT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DMRT1 Antibody (A-9): sc-377167
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DMRT1 CRISPR/Cas9 KO Plasmid (m) | sc-424507 | 20 µg | $397.00 |
Overview
Dmrt1 encodes DMRT1, a conserved transcription factor containing a DM DNA-binding domain that regulates sex determination and differentiation programs in the mouse gonad. DMRT1 is essential for postnatal testis maintenance by promoting Sertoli cell identity, coordinating germ cell development, and repressing inappropriate activation of ovarian pathways, including antagonism of FOXL2-driven networks. Its activity integrates with transcriptional and chromatin-based control of meiosis, spermatogonial differentiation, and somatic–germline signaling within the seminiferous epithelium. Disruption or dysregulation of DMRT1-associated programs is linked to disorders of sex development, impaired spermatogenesis, and gonadal dysgenesis, making it a key node for mechanistic studies of reproductive biology.
DMRT1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dmrt1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dmrt1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dmrt1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DMRT1 protein expression.
This CRISPR knockout system enables efficient generation of Dmrt1-deficient cell models for investigation of DMRT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dmrt1 exon(s) critical for DMRT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dmrt1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DMRT1 CRISPR/Cas9 KO Plasmid (m) and DMRT1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dmrt1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DMRT1 HDR Plasmid (m) and DMRT1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dmrt1 homology arms to support homology-directed repair at defined Dmrt1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.