Anticorps DENR (NQ-B8) recommandé pour la détection de DENR d'origine mouse, rat et human par WB, IP et ELISA

Anticorps DENR (NQ-B8): sc-134315

Anticorps DENR (NQ-B8) est évalué 5.0 de 5 de 1.
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Fiches techniques

    • L'Anticorps DENR (NQ-B8) est un monoclonal de souris IgG2a κ, cité dans 2 publications, fourni en 100 µg/ml
    • raised against recombinant DENR protein of human origin
    • recommandé pour la détection de DENR d'origine mouse, rat et human par WB, IP et ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DENR Antibody (NQ-B8). This work is in progress.
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Hello! I'm interested in this antibody. Why is the band expected at ~28kd, but DENR is a 22kd protein? 

Posée par: YLfhcrc
Thank you for your question. The predicted molecular weight of DENR is 22 kDa, however the observed molecular weight of the endogenous protein is ~27-31 kDa in the cell lines we have tested. The difference between the predicted and observed molecular weight may be due to post-translational modifications of the protein.
Répondue par: Technical Support
Date de publication: 2019-03-10

For Western Blot, is it recommended to use denatured or non-denatured conditions with DENR (NQ-B8): sc-134315 antibody?

Posée par: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Répondue par: Technical Support
Date de publication: 2017-03-27
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Rated 5 de 5 de par WB publishable data with mouse aorta WB publishable data with mouse aorta - SCBT Publication Review
Date de publication: 2014-10-01
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