Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DDX15 Double Nickase Plasmid (h): sc-403959-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX15 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDX15 Double Nickase Plasmid (h) and DDX15 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DHX15. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDX15 Antibody (E-6): sc-271686
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX15 Double Nickase Plasmid (h) | sc-403959-NIC | 20 µg | $410.00 | |||
DDX15 Double Nickase Plasmid (h2) | sc-403959-NIC-2 | 20 µg | $410.00 |
DHX15 (DDX15) encodes a DEAH-box RNA helicase that remodels RNA–protein complexes during pre-mRNA splicing and broader RNA processing events. DDX15 functions within spliceosomal pathways to support intron removal, RNA surveillance, and maturation of transcripts that regulate cell-cycle control and stress-responsive programs. By influencing splice site choice and transcript fidelity, DHX15 can affect proteome composition and signaling outputs across diverse cellular contexts. Dysregulation of RNA helicase activity and splicing factor networks involving DHX15 has been associated with altered gene expression states relevant to proliferative and neurodevelopmental phenotypes, making it a useful node for mechanistic studies of RNA metabolism.
DDX15 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DHX15 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DHX15. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DHX15 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DHX15-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.