Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DDI2 Antibody (A-3) HRP: sc-514004 HRP. Direct western blot analysis of DDI2 expression in HeLa (A), Jurkat (B), Hep G2 (C), BYDP (D), C3H/10T1/2 (E) and 3611-RF (F) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DDI2 CRISPR/Cas9 KO Plasmid (m): sc-427193
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDI2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDI2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DDI2 Antibody (A-3): sc-514004
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDI2 CRISPR/Cas9 KO Plasmid (m) | sc-427193 | 20 µg | $397.00 |