Cytokeratin 5/8 Antibody (C50): sc-8021
- Cytokeratin 5/8 Antibody (C50) is a mouse monoclonal IgG1 κ, cited in 7 publications, provided at 200 µg/ml
- raised against cytoskeleton preparation of HeLa cells
- recommended for detection of Cytokeratin 5/8 of human origin by WB, IP, IF, IHC(P), FCM and ELISA; may cross-react with Cytokeratin 18
- available conjugated to either phycoerythrin, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
- See pan-Cytokeratin (C11): sc-8018 for Cytokeratin 5/8 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cytokeratin 5/8 Antibody (C50) for WB and IHC(P) applications. These reagents are now offered in bundles with Cytokeratin 5/8 Antibody (C50) (see ordering information below).
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Cytokeratin 5/8 Antibody (C50) is a mouse monoclonal IgG1 antibody that detects Cytokeratin 5/8 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Cytokeratin 5/8 plays a vital role in maintaining the structural integrity and mechanical resilience of epithelial cells, which is crucial for the proper functioning of tissues that experience significant stress, such as the skin and respiratory tract. Cytokeratin 5/8 is primarily located in the cytoplasm of epithelial cells, where anti-Cytokeratin 5/8 antibody (C50) detects intermediate filament networks providing support and stability. Cytokeratin 5/8 presence is particularly important in epithelial differentiation and tissue repair, helping to protect cells from mechanical injury and contributing to the overall architecture of epithelial tissues. Alterations in Cytokeratin 5/8 expression can serve as valuable biomarkers for various pathological conditions, including cancers, where expression patterns can indicate tumor differentiation status. Cytokeratin 5 is notably expressed in normal basal cells, and mutations in the gene encoding Cytokeratin 5 (KRT5) are associated with the autosomal dominant disorder epidermolysis bullosa, highlighting this protein′s significance in both health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cytokeratin 5/8 Antibody (C50) References:
- Cytokeratin 8 functions as a major plasminogen receptor in select epithelial and carcinoma cells. | Gonias, SL., et al. 2001. Front Biosci. 6: D1403-11. PMID: 11689350
- Coordinate expression of cytokeratin 8 and cytokeratin 17 immunohistochemical staining in cervical intraepithelial neoplasia and cervical squamous cell carcinoma: an immunohistochemical analysis and review of the literature. | Ikeda, K., et al. 2008. Gynecol Oncol. 108: 598-602. PMID: 18191996
- The Immunohistochemical Expression of the Oestrogen Receptor (ER), HER-2/NEU and Cytokeratin 8/18 and 5/6 in Invasive Breast Carcinoma. | Rattan, B., et al. 2012. J Clin Diagn Res. 6: 1495-8. PMID: 23285439
- Cytokeratin 5-Positive Cells Represent a Therapy Resistant subpopulation in Epithelial Ovarian Cancer. | Corr, BR., et al. 2015. Int J Gynecol Cancer. 25: 1565-73. PMID: 26495758
- Cytokeratin 8/18 protects breast cancer cell lines from TRAIL-induced apoptosis. | Bozza, WP., et al. 2018. Oncotarget. 9: 23264-23273. PMID: 29796187
- Correlation between salivary estrogen levels and oral epithelial cytokeratin 5 expression. | Handajani, J., et al. 2020. F1000Res. 9: 186. PMID: 32399205
- Cytokeratin 5 determines maturation of the mammary myoepithelium. | Deckwirth, V., et al. 2021. iScience. 24: 102413. PMID: 34007958
- Cytokeratin 5 and cytokeratin 6 expressions are unconnected in normal and cancerous tissues and have separate diagnostic implications. | Völkel, C., et al. 2022. Virchows Arch. 480: 433-447. PMID: 34559291
- Clinicopathological features of cytokeratin 5-positive pulmonary adenocarcinoma. | Terada, K., et al. 2023. Histopathology. 82: 439-453. PMID: 36239561
- Cytokeratin 8 depicts nodal metastasis in head and neck squamous cell carcinoma. | Thamilselvan, S., et al. 2024. J Oral Maxillofac Pathol. 28: 247-252. PMID: 39157840
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 5/8 Antibody (C50) | sc-8021 | 200 µg/ml | $322.00 | |||
Cytokeratin 5/8 Antibody (C50): m-IgG Fc BP-HRP Bundle | sc-536561 | 200 µg Ab; 10 µg BP | $361.00 | |||
Cytokeratin 5/8 Antibody (C50): m-IgGκ BP-HRP Bundle | sc-533712 | 200 µg Ab; 40 µg BP | $361.00 | |||
Cytokeratin 5/8 Antibody (C50): m-IgG1 BP-HRP Bundle | sc-544676 | 200 µg Ab; 20 µg BP | $361.00 | |||
Cytokeratin 5/8 Antibody (C50) Alexa Fluor® 488 | sc-8021 AF488 | 200 µg/ml | $364.00 | |||
Cytokeratin 5/8 Antibody (C50) Alexa Fluor® 647 | sc-8021 AF647 | 200 µg/ml | $364.00 | |||
Cytokeratin 5/8 Antibody (C50) PE | sc-8021 PE | 200 µg/ml | $349.00 |