Cytokeratin 18 Antibody (RGE53): sc-32329

Cytokeratin 18 Antibody (RGE53) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cytokeratin 18 in mouse, rat, human, and bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), immunohistochemistry (frozen), and flow cytometry (FCM). Anti-Cytokeratin 18 antibody (RGE53) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cytokeratin 18 is a vital component of the cytoskeleton in epithelial cells, playing a crucial role in maintaining cellular integrity and providing mechanical support. Cytokeratin 18 is predominantly located in the cytoplasm, forming intermediate filaments that contribute to the structural framework of epithelial tissues. Proper localization of Cytokeratin 18 is essential for normal functioning of epithelial cells, helping to resist mechanical stress and facilitate cell-cell adhesion. Alterations in Cytokeratin 18 expression or localization can serve as important biomarkers for various pathological conditions, including cancer, where expression is often upregulated in adenocarcinomas and basal cell carcinomas. Anti-Cytokeratin 18 antibody (RGE53)′s ability to detect across multiple species makes this reagent a valuable tool for researchers studying epithelial biology and disease.

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Cytokeratin 18 Antibody (RGE53) References:

1. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule.  |  Lauerová, L., et al. 1988. Hybridoma. 7: 495-504. PMID: 2461901
2. Monoclonal antibodies recognizing different epitopes of cytokeratin No.18.  |  Vojtĕsek, B., et al. 1989. Folia Biol (Praha). 35: 373-82. PMID: 2483834
3. Serum Cytokeratin 18 as a Metastatic and Therapeutic Marker for Extramammary Paget's Disease.  |  Urano-Takaoka, M., et al. 2022. Acta Derm Venereol. 102: adv00636. PMID: 34904690
4. Serum and urine periostin and cytokeratin-18 in children with congenital obstructive nephropathy.  |  Turczyn, A., et al. 2022. Cent Eur J Immunol. 47: 63-72. PMID: 35600153
5. Hepatocyte apoptosis fragment product cytokeratin-18 M30 level and non-alcoholic steatohepatitis risk diagnosis: an international registry study.  |  Zhang, H., et al. 2023. Chin Med J (Engl). 136: 341-350. PMID: 36848175
6. Evaluation of a new tumor marker for cytokeratin 8 and 18 fragments in healthy individuals and prostate cancer patients.  |  Silén, A., et al. 1994. Prostate. 24: 326-32. PMID: 7516071
7. A novel IRMA and ELISA for quantifying cytokeratin 8 and 18 fragments in the sera of healthy individuals and cancer patients.  |  Silén, A., et al. 1995. Scand J Clin Lab Invest. 55: 153-61. PMID: 7545308
8. Cytokeratin expression in normal and (pre)malignant head and neck epithelia: an overview.  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
9. Serum and tissue distribution of a fragment of cytokeratin 19 (cyfra 21-1) in lung cancer patients.  |  Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
10. Functional inactivation of p53 by antisense RNA induces invasive ability of lung carcinoma cells and downregulates cytokeratin synthesis.  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
11. Cytokeratin expression, fibrillar organization, and subtle function in liver cells.  |  Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681
12. Phosphorylation of human keratin 18 serine 33 regulates binding to 14-3-3 proteins.  |  Ku, NO., et al. 1998. EMBO J. 17: 1892-906. PMID: 9524113

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cytokeratin 18 Antibody (RGE53) has 51 citations in a variety of scientific publications.

Primary antibodies like Anti-Cytokeratin 18 Antibody (RGE53) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cytokeratin 18 Antibody (RGE53) Specifications

Alternate Product Name: KRT18

Product Clone Name: RGE53

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Cytokeratin 18

Epitope Species:

Product Reactivity/Detection: Cytokeratin 18

Species Reactivity/Detection: mouse, rat, human, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IHC(frozen), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 51 citations in various scientific publications

Available Conjugates/Forms for Cytokeratin 18 Antibody (RGE53): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin