Anti-L'anticorps Cytokeratin 15 LHK15 est un monoclonal IgG2a κ L'Anticorps Cytokeratin 15, cité dans 34 publications, fourni en 200 µg/ml
raised against the last 17 amino acids of Cytokeratin 15 of human origin
Anti-L'Anticorps Cytokeratin 15 (LHK15) est recommandé pour la détection de Cytokeratin 15 d'origine mouse, rat et human par WB, IP, IF et IHC(P)
Anti-L'Anticorps Cytokeratin 15 (LHK15) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Cytokeratin 15 (LHK15): sc-47697.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for Cytokeratin 15 Antibody (LHK15) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cytokeratin 15 Antibody (LHK15) (see ordering information below).
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Cytokeratin 15 Antibody (LHK15) is a high quality monoclonal Cytokeratin 15 antibody (also designated KRT15 antibody, or Keratin 15 antibody) suitable for the detection of the Cytokeratin 15 protein of mouse, rat and human origin. Cytokeratin 15 Antibody (LHK15) is available as both the non-conjugated anti-Cytokeratin 15 antibody form, as well as multiple conjugated forms of anti-Cytokeratin 15 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cytokeratin 15 (CK15, K15, K1CO, keratin15, type I cytoskeletal 15) is an intermediate filament (IF) type I protein that is responsible for the mechanical integrity of epithelial cells. Keratin family members are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains, and are clustered in a region on chromosome 17q21.2. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceus. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pricing up to buy this: 200ug/ml, but what volume?
Posée par: Shelly99
Thank you for your question. This is provided as 200 ug in 1 ml volume.
Répondue par: Technical Support
Date de publication: 2020-03-25
Dear SAnta cruz,I wanted to try cytokeratin 15 antibody. But since its mouse antibody i am not sure if it will give me lot of background with my mouse skin sample.Is their any possibility to get free small aliquote for trail Please
Posée par: zumer
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Répondue par: SCBT Heidelberg Support
Date de publication: 2018-12-14
Can Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Posée par: jenni
Thank you for your question. Yes, Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Rated 5 de
works very well in histology alone or in coIFSerial sections from human scalp biopsies and mouse ears embedded in OCT or human scalp biopsies embedded in formalin‐fixed paraffin were processed for immunofluorescence as follow: Frozen sections were fixed with neutral buffered 4% paraformaldehyde (PFA) for 15 min at room temperature (RT) and washed three times with phosphate‐buffered saline (PBS). Paraffin sections were processed for their deparaffinization and antigen retrieval. Primary antibody specific for Keratin 15 at 1:400 dilution was incubated overnight (O/N) at 4°C. Sections were then washed three times with PBS and incubated with secondary antibody conjugated to the appropriate fluorophore(s) (for example, Alexa Fluor 647 dilution 1:200) for 1 h at RT. Sections were then washed three times with PBS, counterstained with DAPI, mounted with IF-grate antifade mounting medium and analyzed by fluorescence or confocal microscopy.
Date de publication: 2019-12-04
Rated 4 de
good antibodythe antibody was tested for flow cytometry in mammary cells. worked well.
Date de publication: 2017-11-23
Rated 5 de
Outstanding IHC stainingGreat product for IHC staining applications; also nice WB
Date de publication: 2017-01-14
Rated 5 de
Great IHC(P) dataThe Cytokeratin 15 Antibody (LHK15): sc-47697 works very well for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has been cited over 10 times for further verification.
Date de publication: 2017-01-10
Rated 5 de
Excellent images obtained in IF with humanExcellent images obtained in IF with human placenta -SCBT Publication Review
Date de publication: 2015-03-07
Rated 5 de
Works well in IHC with paraffinWorks well in IHC with paraffin-embedded human fetal corneal cells -SCBT Publication Review
Date de publication: 2015-03-06
Rated 3 de
Good single band with some backgroundGood single band with some background -SCBT QC
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