Anticorps Cytokeratin 15 (LHK15) recommandé pour la détection de Cytokeratin 15 d'origine mouse, rat et human par WB, IP, IF et IHC(P)

Anticorps Cytokeratin 15 (LHK15): sc-47697

Anticorps Cytokeratin 15 (LHK15) est évalué 4.6 de 5 de 7.
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Fiches techniques

    • Anti-L'anticorps Cytokeratin 15 LHK15 est un monoclonal IgG2a κ L'Anticorps Cytokeratin 15, cité dans 34 publications, fourni en 200 µg/ml
    • raised against the last 17 amino acids of Cytokeratin 15 of human origin
    • Anti-L'Anticorps Cytokeratin 15 (LHK15) est recommandé pour la détection de Cytokeratin 15 d'origine mouse, rat et human par WB, IP, IF et IHC(P)
    • Anti-L'Anticorps Cytokeratin 15 (LHK15) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Cytokeratin 15 (LHK15): sc-47697.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for Cytokeratin 15 Antibody (LHK15) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cytokeratin 15 Antibody (LHK15) (see ordering information below).

Pricing up to buy this:  200ug/ml,  but what volume?

Posée par: Shelly99
Thank you for your question. This is provided as 200 ug in 1 ml volume.
Répondue par: Technical Support
Date de publication: 2020-03-25

Dear SAnta cruz,I wanted to try cytokeratin 15 antibody. But since its mouse antibody i am not sure if it will give me lot of background with my mouse skin sample.Is their any possibility to get free small aliquote for trail Please

Posée par: zumer
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Répondue par: SCBT Heidelberg Support
Date de publication: 2018-12-14

Can Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: jenni
Thank you for your question. Yes, Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support 8
Date de publication: 2017-01-10
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Rated 5 de 5 de par works very well in histology alone or in coIF Serial sections from human scalp biopsies and mouse ears embedded in OCT or human scalp biopsies embedded in formalin‐fixed paraffin were processed for immunofluorescence as follow: Frozen sections were fixed with neutral buffered 4% paraformaldehyde (PFA) for 15 min at room temperature (RT) and washed three times with phosphate‐buffered saline (PBS). Paraffin sections were processed for their deparaffinization and antigen retrieval. Primary antibody specific for Keratin 15 at 1:400 dilution was incubated overnight (O/N) at 4°C. Sections were then washed three times with PBS and incubated with secondary antibody conjugated to the appropriate fluorophore(s) (for example, Alexa Fluor 647 dilution 1:200) for 1 h at RT. Sections were then washed three times with PBS, counterstained with DAPI, mounted with IF-grate antifade mounting medium and analyzed by fluorescence or confocal microscopy.
Date de publication: 2019-12-04
Rated 4 de 5 de par good antibody the antibody was tested for flow cytometry in mammary cells. worked well.
Date de publication: 2017-11-23
Rated 5 de 5 de par Outstanding IHC staining Great product for IHC staining applications; also nice WB
Date de publication: 2017-01-14
Rated 5 de 5 de par Great IHC(P) data The Cytokeratin 15 Antibody (LHK15): sc-47697 works very well for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has been cited over 10 times for further verification.
Date de publication: 2017-01-10
Rated 5 de 5 de par Excellent images obtained in IF with human Excellent images obtained in IF with human placenta -SCBT Publication Review
Date de publication: 2015-03-07
Rated 5 de 5 de par Works well in IHC with paraffin Works well in IHC with paraffin-embedded human fetal corneal cells -SCBT Publication Review
Date de publication: 2015-03-06
Rated 3 de 5 de par Good single band with some background Good single band with some background -SCBT QC
Date de publication: 2015-02-15
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