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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CYP27A1 Antibody (G-2) is a high quality monoclonal CYP27A1 antibody (also designated CYP27A1 antibody) suitable for the detection of the CYP27A1 protein of mouse, rat and human origin. CYP27A1 Antibody (G-2) is available as both the non-conjugated anti-CYP27A1 antibody form, as well as multiple conjugated forms of anti-CYP27A1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endo-genous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. Other P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased aromatase activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7a-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rated 5 de
Cyp27a1 antibody gives good bands on Liver WesternReceived a Cyp27a1 sample antibody with a Cyp8b1 sample antibody for the revision of one of my manuscripts. And both worked really well with mouse liver samples on westerns. I loaded 20-50mcg protein, used 1:1000 dilution of primary (Cyp27a1 and Cyp8b1 respectively) and then secondary antibody each, and developed with a "femto" enhancer instead of a "pico" (=regular enhancer) which I commonly do, and I got nice strong bands. I know many people use lower concentrations of primary antibody (which should work as well I think); but we tend to use these concentrations in our lab. Bottom line: This Cyp27a1 antibody works well, and I am happy that it did in my case, as I had several other experiments to complete for my revision.
Date de publication: 2017-09-21
Rated 1 de
Non adatto ai campioni/metodiche utilizzatiBuongiorno,
ho testato CYP27A1 e CYP46A1 su cervelli umani fissati in PFA 4% ed inclusi in paraffina, sia in IIC che IF, ma non ho avuto risultati soddisfacenti.
Date de publication: 2017-08-28
Rated 4 de
Stephen Curry par
Strong CYP27A1 WB signals made in our labStrong CYP27A1 WB signals have been made with human sample in our lab
Date de publication: 2017-02-16
Rated 5 de
strong band at 55kDThere was a strong band at 55kD in human samples by WB
Date de publication: 2016-12-14
Rated 5 de
Robust signal obtained via Western Blot using HUVRobust signal obtained via Western Blot using HUV-EC-C, HepG2 and CaCo-2 whole cell lysates and human liver tissue extract. -SCBT QC
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