Anticorps CYP27A1 (G-2) recommandé pour la détection de CYP27A1 d'origine mouse, rat et human par WB, IP, IF et ELISA

Anticorps CYP27A1 (G-2): sc-390974

Anticorps CYP27A1 (G-2) est évalué 4.0 de 5 de 5.
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Fiches techniques

    • Anti-L'anticorps CYP27A1 G-2 est un monoclonal IgG2a (chaîne légère kappa) L'Anticorps CYP27A1 fourni en 200 µg/ml
    • dirigé contre les acides aminés 335-451 dans une région interne de CYP27A1 d'origine human
    • Anti-L'Anticorps CYP27A1 (G-2) est recommandé pour la détection de CYP27A1 d'origine mouse, rat et human par WB, IP, IF et ELISA
    • Anti-L'Anticorps CYP27A1 (G-2) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CYP27A1 (G-2): sc-390974.
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I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-390974: CYP27A1 (G-2) mouse monoclonal antibody will work?

Posée par: TinTin
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Répondue par: Technical Support
Date de publication: 2017-03-06
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Rated 5 de 5 de par Cyp27a1 antibody gives good bands on Liver Western Received a Cyp27a1 sample antibody with a Cyp8b1 sample antibody for the revision of one of my manuscripts. And both worked really well with mouse liver samples on westerns. I loaded 20-50mcg protein, used 1:1000 dilution of primary (Cyp27a1 and Cyp8b1 respectively) and then secondary antibody each, and developed with a "femto" enhancer instead of a "pico" (=regular enhancer) which I commonly do, and I got nice strong bands. I know many people use lower concentrations of primary antibody (which should work as well I think); but we tend to use these concentrations in our lab. Bottom line: This Cyp27a1 antibody works well, and I am happy that it did in my case, as I had several other experiments to complete for my revision.
Date de publication: 2017-09-21
Rated 1 de 5 de par Non adatto ai campioni/metodiche utilizzati Buongiorno, ho testato CYP27A1 e CYP46A1 su cervelli umani fissati in PFA 4% ed inclusi in paraffina, sia in IIC che IF, ma non ho avuto risultati soddisfacenti. Cordiali saluti, Alessandra Milesi
Date de publication: 2017-08-28
Rated 4 de 5 de par Strong CYP27A1 WB signals made in our lab Strong CYP27A1 WB signals have been made with human sample in our lab
Date de publication: 2017-02-16
Rated 5 de 5 de par strong band at 55kD There was a strong band at 55kD in human samples by WB
Date de publication: 2016-12-14
Rated 5 de 5 de par Robust signal obtained via Western Blot using HUV Robust signal obtained via Western Blot using HUV-EC-C, HepG2 and CaCo-2 whole cell lysates and human liver tissue extract. -SCBT QC
Date de publication: 2014-03-03
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