cyclin T1 Antibody (C-6): sc-271575
- cyclin T1 Antibody (C-6) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 261-505 mapping within an internal region of cyclin T1 of human origin
- Anti-cyclin T1 Antibody (C-6) is recommended for detection of cyclin T1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-cyclin T1 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-271575 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for cyclin T1 Antibody (C-6) for WB applications. This reagent is now offered in a bundle with cyclin T1 Antibody (C-6) (see ordering information below).
QUICK LINKS
Cyclin T1 Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects cyclin T1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-cyclin T1 antibody (C-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cyclin T1 plays a crucial role in regulating transcription elongation by forming a complex with cyclin-dependent kinase 9 (Cdk9), which is essential for phosphorylating the C-terminal domain of RNA polymerase II. This phosphorylation enables RNA polymerase II to transition from transcription initiation to elongation, facilitating mRNA production. Cyclin T1 also interacts with HIV-1 Tat protein, enhancing its binding affinity to the viral TAR RNA structure, which is critical for recruiting the cyclin T1/Cdk9 complex to RNA polymerase II. The specificity of cyclin T1 in this interaction highlights its importance in both cellular transcription processes and viral pathogenesis, making cyclin T1 a significant target for research in gene expression and HIV biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cyclin T1 Antibody (C-6) References:
- Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. | Marshall, RM., et al. 2005. J Immunol. 175: 6402-11. PMID: 16272292
- Cyclin T1 but not cyclin T2a is induced by a post-transcriptional mechanism in PAMP-activated monocyte-derived macrophages. | Liou, LY., et al. 2006. J Leukoc Biol. 79: 388-96. PMID: 16330531
- Cyclin box structure of the P-TEFb subunit cyclin T1 derived from a fusion complex with EIAV tat. | Anand, K., et al. 2007. J Mol Biol. 370: 826-36. PMID: 17540406
- Cyclin T1-dependent genes in activated CD4 T and macrophage cell lines appear enriched in HIV-1 co-factors. | Yu, W., et al. 2008. PLoS One. 3: e3146. PMID: 18773076
- Cyclin T1 overexpression induces malignant transformation and tumor growth. | Moiola, C., et al. 2010. Cell Cycle. 9: 3119-26. PMID: 20714219
- Regulation of cyclin T1 and HIV-1 Replication by microRNAs in resting CD4+ T lymphocytes. | Chiang, K., et al. 2012. J Virol. 86: 3244-52. PMID: 22205749
- Regulation of the Alternative Splicing and Function of Cyclin T1 by the Serine-Arginine-Rich Protein ASF/SF2. | Zhou, J., et al. 2017. J Cell Biochem. 118: 4020-4032. PMID: 28422315
- Reversible phosphorylation of cyclin T1 promotes assembly and stability of P-TEFb. | Huang, F., et al. 2021. Elife. 10: PMID: 34821217
- Disrupting the Cdk9/Cyclin T1 heterodimer of 7SK snRNP for the Brd4 and AFF1/4 guided reconstitution of active P-TEFb. | Zhou, K., et al. 2022. Nucleic Acids Res. 50: 750-762. PMID: 34935961
- Discovery of a tetrahydroisoquinoline-based CDK9-cyclin T1 protein-protein interaction inhibitor as an anti-proliferative and anti-migration agent against triple-negative breast cancer cells. | Cheng, S., et al. 2022. Genes Dis. 9: 1674-1688. PMID: 36157485
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin T1 Antibody (C-6) | sc-271575 | 200 µg/ml | $322.00 | |||
cyclin T1 Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-525815 | 200 µg Ab; 10 µg BP | $361.00 | |||
cyclin T1 Antibody (C-6) X | sc-271575 X | 200 µg/0.1 ml | $322.00 | |||
cyclin T1 Antibody (C-6) AC | sc-271575 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cyclin T1 Antibody (C-6) HRP | sc-271575 HRP | 200 µg/ml | $322.00 | |||
cyclin T1 Antibody (C-6) FITC | sc-271575 FITC | 200 µg/ml | $336.00 | |||
cyclin T1 Antibody (C-6) PE | sc-271575 PE | 200 µg/ml | $349.00 | |||
cyclin T1 Antibody (C-6) Alexa Fluor® 488 | sc-271575 AF488 | 200 µg/ml | $364.00 | |||
cyclin T1 Antibody (C-6) Alexa Fluor® 546 | sc-271575 AF546 | 200 µg/ml | $364.00 | |||
cyclin T1 Antibody (C-6) Alexa Fluor® 594 | sc-271575 AF594 | 200 µg/ml | $364.00 | |||
cyclin T1 Antibody (C-6) Alexa Fluor® 647 | sc-271575 AF647 | 200 µg/ml | $364.00 | |||
cyclin T1 Antibody (C-6) Alexa Fluor® 680 | sc-271575 AF680 | 200 µg/ml | $364.00 | |||
cyclin T1 Antibody (C-6) Alexa Fluor® 790 | sc-271575 AF790 | 200 µg/ml | $364.00 |