cyclin A Antibody (E72.1): sc-53232

Cyclin A Antibody (E72.1) is a mouse monoclonal IgG1 antibody that detects cyclin A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Cyclin A plays a pivotal role in regulating the eukaryotic cell cycle, particularly during S phase, where cyclin A is crucial for proper DNA replication progression. Cyclin A is primarily located in the nucleus, where cyclin A associates with cyclin-dependent kinase-2 (Cdk2) to form active complexes driving cell cycle transitions. Nuclear localization of cyclin A ensures precise cell cycle machinery regulation, preventing uncontrolled cell division that can lead to tumorigenesis. Cyclin A has been implicated in various cellular processes, including cell transformation, and interacts with key regulatory proteins such as E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein, p110. Anti-cyclin A antibody (E72.1) serves as an essential tool for researchers studying cell cycle dynamics and molecular mechanisms underlying cell proliferation and cancer.

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cyclin A Antibody (E72.1) References:

1. DNA damage leads to a Cyclin A-dependent delay in metaphase-anaphase transition in the Drosophila gastrula.  |  Su, TT. and Jaklevic, B. 2001. Curr Biol. 11: 8-17. PMID: 11166174
2. C/EBPalpha is required for proteolytic cleavage of cyclin A by calpain 3 in myeloid precursor cells.  |  Welm, AL., et al. 2002. J Biol Chem. 277: 33848-56. PMID: 12105198
3. Alterations in cyclin A, B, and D1 in mouse dentate gyrus following TMT-induced hippocampal damage.  |  McPherson, CA., et al. 2003. Neurotox Res. 5: 339-54. PMID: 14715453
4. Prognostic significance of cyclin A in gastric cancer.  |  Mrena, J., et al. 2006. Int J Cancer. 119: 1897-901. PMID: 16708383
5. Direct association of the HPV16 E7 oncoprotein with cyclin A/CDK2 and cyclin E/CDK2 complexes.  |  Nguyen, CL. and Münger, K. 2008. Virology. 380: 21-5. PMID: 18718623
6. Combination of the proliferation marker cyclin A, histological grade, and estrogen receptor status in a new variable with high prognostic impact in breast cancer.  |  Strand, C., et al. 2012. Breast Cancer Res Treat. 131: 33-40. PMID: 21331623
7. A novel arylbenzofuran induces cervical cancer cell apoptosis and G1/S arrest through ERK-mediated Cdk2/cyclin-A signaling pathway.  |  Ming, P., et al. 2016. Oncotarget. 7: 41843-41856. PMID: 27259234
8. Cyclin A-cdk1-Dependent Phosphorylation of Bora Is the Triggering Factor Promoting Mitotic Entry.  |  Vigneron, S., et al. 2018. Dev Cell. 45: 637-650.e7. PMID: 29870721
9. Cyclin A/B RxL Macrocyclic Inhibitors to Treat Cancers with High E2F Activity.  |  Singh, S., et al. 2024. bioRxiv.. PMID: 39211113
10. Activation of the rat cyclin A promoter by ATF2 and Jun family members and its suppression by ATF4.  |  Shimizu, M., et al. 1998. Exp Cell Res. 239: 93-103. PMID: 9511728

The preferred cyclin A antibody is cyclin A Antibody (BF683): sc-239.