Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CUL-3 CRISPR/Cas9 KO Plasmid (h): sc-416925
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CUL-3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CUL-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CUL-3 Antibody (G-8): sc-166110
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CUL-3 CRISPR/Cas9 KO Plasmid (h) | sc-416925 | 20 µg | $397.00 |
Overview
CUL3 encodes cullin-3 (CUL-3), a core scaffold of CUL3–RBX1 E3 ubiquitin ligase complexes that recruit BTB-domain adaptor proteins to confer substrate specificity for ubiquitination and proteasomal turnover. Through regulated degradation of targets involved in redox homeostasis, cell-cycle control, cytoskeletal dynamics, and stress signaling, CUL-3 helps coordinate pathways such as ubiquitin–proteasome function and NRF2/KEAP1-mediated oxidative stress responses. Perturbation of CUL3-dependent ubiquitination can disrupt proteostasis and signaling networks that shape inflammation, metabolism, and genome integrity. Dysregulated CUL3 activity and altered adaptor interactions have been linked in the literature to cancer-associated pathways and cardiovascular and neurodevelopmental disease mechanisms, supporting its utility as a node for pathway dissection.
CUL-3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CUL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CUL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CUL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CUL-3 protein expression.
This CRISPR knockout system enables efficient generation of CUL3-deficient cell models for investigation of CUL-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CUL3 exon(s) critical for CUL-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CUL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CUL-3 CRISPR/Cas9 KO Plasmid (h) and CUL-3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CUL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CUL-3 HDR Plasmid (h) and CUL-3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CUL3 homology arms to support homology-directed repair at defined CUL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.