Date published: 2025-9-7

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Anticorps CstF-77 (F-3): sc-376553

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Fiches techniques
  • L'Anticorps CstF-77 (F-3) est un monoclonal de souris IgG1 κ, cité dans 1 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 31-330 situés près de la région N-terminus de CstF-77 d'origine human
  • recommandé pour la détection de CstF-77 d'origine mouse, rat, human et avian par WB, IP, IF et ELISA; également réactif avec d'autres espèces, dont et canine, bovine and avian
  • m-IgG Fc BP-HRP et 1 BP-HRP">m-IgG1 BP-HRP sont les réactifs de détection secondaire préférés pour CstF-77 Antibody (F-3) for WB applications. Ces réactifs sont désormais proposés en lots avec CstF-77 Antibody (F-3)(voir les informations de commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. CstF-77 (cleavage stimulation factor, 77 kDa subunit), also known as CstF3, is one of the three subunits comprising CstF. It can exist as a homodimer and functions as the bridge, directly interacting with the other two CstF subunits, namely CstF-64 and CstF-50. CstF-77 is highly conserved among eukaryotes. It contains an α-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly. In addition, CstF-77 is capable of interacting with CPSF1 and FCP1, other factors involved in polyadenylation.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps CstF-77 (F-3)

sc-376553
200 µg/ml
$316.00

CstF-77 (F-3): m-IgG Fc BP-HRP Kit

sc-540598
200 µg Ab; 10 µg BP
$354.00

CstF-77 (F-3): m-IgG1 BP-HRP Kit

sc-542233
200 µg Ab; 20 µg BP
$354.00

What is the recommended fixation method for immunofluorescence staining with CstF-77 (F-3): sc-376553 monoclonal antibody?

Posée par: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-03-01
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Rated 5 de 5 de par Excellent immunofluorescence nuclear stainingExcellent immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date de publication: 2015-04-12
Rated 5 de 5 de par Great Western Blot data of CstFGreat Western Blot data of CstF-77 expression in HL-60 and Jurkat whole cell lysates. -SCBT QC
Date de publication: 2013-09-19
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Anticorps CstF-77 (F-3) est évalué 5.0 de 5 de 2.
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