Date published: 2025-9-10

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Anticorps cSHMT (S-W6): sc-100849

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Fiches techniques
  • L'Anticorps cSHMT (S-W6) est un monoclonal de souris IgG1 κ, cité dans 1 publications, fourni en 100 µg/ml
  • dirigé contre la cSHMT recombinante d'origine human
  • recommandé pour la détection de cytosolic SHMT d'origine human par WB, IP, IF, IHC(P) et ELISA
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire cSHMT (S-W6). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Mammalian serine hydroxymethyltransferase (SHMT) is a tetrameric, pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the reversible interconversion of serine and tetrahydrofolate to glycine and methylenetetrahydrofolate in the cytoplasm (cSHMT, SHMT1) and mitochondria (mSHMT, SHMT2). cSHMT preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate, and inhibits SAM synthesis. Sheep liver cytosolic recombinant SHMT (scSHMT) Lys-71, Arg-80 and Asp 89 residues influence intra-subunit ionic interactions essential for catalytic activity; Tyr 72, Asp 227 and His-356 residues in the active site interact with PLP and maintain the tetrameric structure. Human cSHMT and mSHMT genes map to 17p11.2 and 12q13, respectively. The cDNA for the mitochondrial enzyme encodes a mature protein of 474 residues.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps cSHMT (S-W6)

sc-100849
100 µg/ml
$333.00

Can sc-100849: cSHMT (S-W6) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Posée par: Germaine
Thank you for your question. Yes, sc-100849: cSHMT (S-W6) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
  • y_2025, m_9, d_7, h_4CST
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Rated 4 de 5 de par Clear immunoperoxidase cytoplasmic stainingClear immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human colon tissue. -SCBT QC
Date de publication: 2015-02-16
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Anticorps cSHMT (S-W6) est évalué 4.0 de 5 de 2.
  • y_2025, m_9, d_7, h_4
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