CRABP-I CRISPR/Cas9 KO Plasmid (m): sc-419790
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRABP-I CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CRABP-I genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRABP-I CRISPR/Cas9 KO Plasmid (m) | sc-419790 | 20 µg | $397.00 |
Overview
Crabp1 encodes cellular retinoic acid–binding protein 1 (CRABP-I), a high-affinity intracellular carrier that buffers and traffics all-trans retinoic acid to shape retinoid availability within the cytosol and nucleus. By modulating retinoic acid partitioning and metabolism, CRABP-I influences RA-dependent transcriptional programs that control cell fate decisions, differentiation, and tissue patterning. In mouse systems, Crabp1 is used to dissect context-specific retinoid signaling dynamics distinct from canonical RAR/RXR activation, including interactions with RA catabolism and cellular responsiveness to vitamin A–derived cues. Altered retinoid handling is broadly relevant to developmental phenotypes and disease-associated dysregulation of differentiation and homeostasis, making Crabp1 a useful node for pathway-level perturbation studies.
CRABP-I CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Crabp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Crabp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Crabp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CRABP-I protein expression.
This CRISPR knockout system enables efficient generation of Crabp1-deficient cell models for investigation of CRABP-I signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Crabp1 exon(s) critical for CRABP-I function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Crabp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CRABP-I CRISPR/Cas9 KO Plasmid (m) and CRABP-I CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Crabp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CRABP-I HDR Plasmid (m) and CRABP-I HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Crabp1 homology arms to support homology-directed repair at defined Crabp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.