Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
COL2A1 CRISPR/Cas9 KO Plasmid (h): sc-400359
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL2A1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COL2A1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COL2A1 Antibody (M2139): sc-52658
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL2A1 CRISPR/Cas9 KO Plasmid (h) | sc-400359 | 20 µg | $397.00 |
Overview
COL2A1 encodes the α1 chain of type II collagen, a major fibrillar collagen that forms the structural framework of hyaline cartilage and contributes to the biomechanical properties of the extracellular matrix. Through assembly into collagen II fibrils and interactions with proteoglycans, COL2A1 supports chondrocyte differentiation, matrix organization, and cartilage homeostasis during skeletal development. COL2A1 expression is regulated by chondrogenic transcriptional programs such as SOX9-driven pathways and coordinates with collagen processing, secretion, and extracellular matrix remodeling networks. Genetic disruption or dysregulation of COL2A1 is linked to a spectrum of heritable cartilage and skeletal disorders, making it a key target for studying extracellular matrix biology and chondrocyte pathology.
COL2A1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COL2A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COL2A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COL2A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COL2A1 protein expression.
This CRISPR knockout system enables efficient generation of COL2A1-deficient cell models for investigation of COL2A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COL2A1 exon(s) critical for COL2A1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COL2A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COL2A1 CRISPR/Cas9 KO Plasmid (h) and COL2A1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COL2A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COL2A1 HDR Plasmid (h) and COL2A1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COL2A1 homology arms to support homology-directed repair at defined COL2A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.