COL15A1 Double Nickase Plasmid (h): sc-404027-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL15A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COL15A1 Double Nickase Plasmid (h) and COL15A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COL15A1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL15A1 Double Nickase Plasmid (h) | sc-404027-NIC | 20 µg | $410.00 | |||
COL15A1 Double Nickase Plasmid (h2) | sc-404027-NIC-2 | 20 µg | $410.00 |
COL15A1 encodes collagen type XV alpha 1 chain, a non-fibrillar basement membrane–associated collagen that contributes to extracellular matrix organization and mechanical stability of microvascular and pericellular niches. COL15A1 supports cell–matrix adhesion, regulates tissue architecture, and influences processes such as angiogenesis and stromal remodeling through integrin-linked signaling and basement membrane assembly. Altered COL15A1 expression and ECM composition are implicated in fibrosis-associated remodeling and in tumor microenvironment biology, where collagen networks can modulate invasion, vascular integrity, and cell migration. As a matrix component with context-dependent expression, COL15A1 is frequently used to study ECM–cell crosstalk and niche-dependent regulation of epithelial and endothelial phenotypes.
COL15A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COL15A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COL15A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COL15A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COL15A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.