CLIP-170 Double Nickase Plasmid (m): sc-425158-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLIP-170 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLIP-170 Double Nickase Plasmid (m) and CLIP-170 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Clip1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLIP-170 Antibody (F-3): sc-28325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLIP-170 Double Nickase Plasmid (m) | sc-425158-NIC | 20 µg | $410.00 | |||
CLIP-170 Double Nickase Plasmid (m2) | sc-425158-NIC-2 | 20 µg | $410.00 |
Mouse Clip1 encodes CLIP-170, a microtubule plus-end tracking protein that binds end-binding proteins and links dynamic microtubules to cortical and organelle-associated factors. CLIP-170 regulates microtubule polymerization, kinetochore capture, and microtubule-dependent vesicle trafficking, influencing cell polarity, migration, and mitotic progression. Through coordination of cytoskeletal remodeling and intracellular transport, CLIP-170 helps shape signaling outputs that depend on spatial organization, including pathways controlling adhesion and spindle checkpoint fidelity. Dysregulation of microtubule dynamics and trafficking-associated processes is broadly relevant to proliferative and neurodevelopmental phenotypes, making Clip1 a useful target for mechanistic studies of cytoskeletal control.
CLIP-170 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Clip1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Clip1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Clip1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Clip1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.