Anti-L'anticorps CKR-5 D-6 est un monoclonal IgG1 κ L'Anticorps CKR-5, cité dans 13 publications, fourni en 200 µg/ml
dirigé contre les acides aminés 66-250 de C-C chemokine receptor gene 5 (CKR-5) d'origine human
Anti-L'Anticorps CKR-5 (D-6) est recommandé pour la détection de CKR-5 d'origine mouse, rat et human par WB, IP, IF et ELISA
Anti-L'Anticorps CKR-5 (D-6) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CKR-5 (D-6): sc-17833.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for CKR-5 Antibody (D-6) for WB applications. These reagents are now offered in bundles with CKR-5 Antibody (D-6) (see ordering information below).
Les Produits Chimiques ChemCruz™
CKR-5 Inhibiteurs pour l'analyse fonctionnelle des réponses cellulaires de C-C Chemokine Receptor
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CKR-5 Antibody (D-6) is a high quality monoclonal CKR-5 antibody (also designated CCR5 antibody or CMKBR5 antibody) suitable for the detection of the CKR-5 protein of mouse, rat and human origin. CKR-5 Antibody (D-6) is available as both the non-conjugated anti-CKR-5 antibody form, as well as multiple conjugated forms of anti-CKR-5 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. C-C or β chemokine family members are characterized by a pair of adjacent cysteine residues and serve as potent chemoattractants and activators of monocytes and T cells. C-C chemokine receptor family members include CKR-1, CKR-2A, CKR-2B, CKR-3, CKR-4, CKR-5, CKR-6, CKR-7, CKR-8, CKR-9, CKR-10 and the Duffy blood group antigen. Each of these receptors are G protein-coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation. However, this receptor family has also been shown to facilitate viral infection. Termed a "co-receptor", CKR-5, along with CD4, has been shown to be a major receptor for HIV. CKR-5 tends to associate with macrophage-tropic viruses, such as macrophage tropic HIV-1, while CKR-2B and CKR-3 bind a minority of viruses.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What are the exact conditions used to obtain the Western blot data shown on your website for CKR-5 (D-6): sc-17833 monoclonal antibody?
Posée par: cjMara
Thank you for your question. We used this primary antibody at a dilution of 1:200 and incubated for one hour at room temperature in Blotto (sc-2333). The complete recommended protocol for Western blotting is provided below.
Rated 5 de
Date de publication: 2018-06-18
Rated 1 de
anti-CKR5 Ab is not working (very non-specific)Initially, I was having trouble determining the right band for CCR5 on the WB from all the other non-specific bands. Therefore, I decided to run true negative control. I used tzm-bl cell (positive for CCR5 expression) and Hela cells (negative control) to chekc any differences in banding pattern and I do not see any difference. There should not be any CCR5 band in Hela cells.
Date de publication: 2018-04-12
Rated 4 de
Clear with exact positionDiluted 1:500 with casein buffer, samples from ACHN cell line, PVDF memberane with 300mA 120min transfering. It is easy to scan with Odyssey system. The CKR5 is located at about 46 kDa, however, there are two non-specific bands above it.
Date de publication: 2018-01-26
Rated 3 de
Zhi Wang par
some non-specific banduse the monoclone antibody with dilution of 1:500, I can detect the CCR5 band successfully, but there are still some non-specific band on the membrane.
Date de publication: 2018-01-02
Rated 1 de
WBThere was a clear band of WB sample. But its molecular weight (~25 kD) looked smaller than predicted molecular weight (46 kD).
Date de publication: 2017-09-10
Rated 5 de
Highly recommended for WBWwell characterized for WB in human, mouse and rat samples.
Date de publication: 2016-12-19
Rated 5 de
very good antibody for our Western blot asvery good antibody for our Western blot as well as for IP
Date de publication: 2015-10-21
Rated 5 de
No issuesNo issues, very good antibody for our Western.
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