Anticorps CKR-5 (D-6) recommandé pour la détection de CKR-5 d'origine mouse, rat et human par WB, IP, IF et ELISA

Anticorps CKR-5 (D-6): sc-17833

Anticorps CKR-5 (D-6) est évalué 3.8 de 5 de 13.
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Fiches techniques

    • Anti-L'anticorps CKR-5 D-6 est un monoclonal IgG1 κ L'Anticorps CKR-5, cité dans 13 publications, fourni en 200 µg/ml
    • dirigé contre les acides aminés 66-250 de C-C chemokine receptor gene 5 (CKR-5) d'origine human
    • Anti-L'Anticorps CKR-5 (D-6) est recommandé pour la détection de CKR-5 d'origine mouse, rat et human par WB, IP, IF et ELISA
    • Anti-L'Anticorps CKR-5 (D-6) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CKR-5 (D-6): sc-17833.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for CKR-5 Antibody (D-6) for WB applications. These reagents are now offered in bundles with CKR-5 Antibody (D-6) (see ordering information below).

What are the exact conditions used to obtain the Western blot data shown on your website for CKR-5 (D-6): sc-17833 monoclonal antibody?

Posée par: cjMara
Thank you for your question. We used this primary antibody at a dilution of 1:200 and incubated for one hour at room temperature in Blotto (sc-2333). The complete recommended protocol for Western blotting is provided below. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Répondue par: Technical Support
Date de publication: 2017-01-27
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Rated 5 de 5 de par 非常好用 选择抗体的时候,不必对了几个厂家最后选择这会款,用于检测CCR5,大鼠,wB用,效果挺好的,非常满意,推荐购买。
Date de publication: 2018-06-18
Rated 1 de 5 de par anti-CKR5 Ab is not working (very non-specific) Initially, I was having trouble determining the right band for CCR5 on the WB from all the other non-specific bands. Therefore, I decided to run true negative control. I used tzm-bl cell (positive for CCR5 expression) and Hela cells (negative control) to chekc any differences in banding pattern and I do not see any difference. There should not be any CCR5 band in Hela cells.
Date de publication: 2018-04-12
Rated 4 de 5 de par Clear with exact position Diluted 1:500 with casein buffer, samples from ACHN cell line, PVDF memberane with 300mA 120min transfering. It is easy to scan with Odyssey system. The CKR5 is located at about 46 kDa, however, there are two non-specific bands above it.
Date de publication: 2018-01-26
Rated 3 de 5 de par some non-specific band use the monoclone antibody with dilution of 1:500, I can detect the CCR5 band successfully, but there are still some non-specific band on the membrane.
Date de publication: 2018-01-02
Rated 1 de 5 de par WB There was a clear band of WB sample. But its molecular weight (~25 kD) looked smaller than predicted molecular weight (46 kD).
Date de publication: 2017-09-10
Rated 5 de 5 de par Highly recommended for WB Wwell characterized for WB in human, mouse and rat samples.
Date de publication: 2016-12-19
Rated 5 de 5 de par very good antibody for our Western blot as very good antibody for our Western blot as well as for IP
Date de publication: 2015-10-21
Rated 5 de 5 de par No issues No issues, very good antibody for our Western.
Date de publication: 2015-08-13
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