CD68 Antibody (KP1): sc-20060

CD68 Antibody (KP1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD68 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and flow cytometry (FCM). CD68 (KP1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD68 protein, homologous to mouse antigen macrosialin, belongs to a family of acidic, highly glycosylated lysosomal glycoproteins including lamp-1 and lamp-2. CD68 (KP1) monoclonal antibody recognizes a protein that plays a crucial role in the immune system, primarily functioning as a marker for macrophages and other myeloid cells essential for phagocytosis and cellular debris clearance. CD68 protein predominantly localizes in cytoplasmic granules and cytoplasm of various non-hematopoietic tissues, such as liver and kidney tubules and glomeruli, and appears on the surface of macrophages, monocytes, neutrophils, basophils, and large lymphocytes. CD68 presence on these immune cells is vital for activation and function, aiding in pathogen recognition and uptake of apoptotic cells, thereby contributing to body′s defense mechanisms and tissue homeostasis.

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CD68 Antibody (KP1) References:

1. Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking.  |  Fukuda, M. 1991. J Biol Chem. 266: 21327-30. PMID: 1939168
2. Distribution of the CD68 macrophage/myeloid associated antigen.  |  Pulford, KA., et al. 1990. Int Immunol. 2: 973-80. PMID: 2078523
3. Expression of the CD68 glycoprotein in the rat epididymis.  |  Liguori, G., et al. 2015. Biochimie. 118: 221-4. PMID: 26433032
4. CD68 expression in the placenta of gestational diabetic mothers: A case-control study.  |  Gosain, R., et al. 2023. Indian J Pathol Microbiol. 66: 727-731. PMID: 38084523
5. Intraplaque Neovascularization, CD68+ and iNOS2+ Macrophage Infiltrate Intensity Are Associated with Atherothrombosis and Intraplaque Hemorrhage in Severe Carotid Atherosclerosis.  |  Balmos, IA., et al. 2023. Biomedicines. 11: PMID: 38137496
6. Unraveling the potential of CD8, CD68, and VISTA as diagnostic and prognostic markers in patients with pancreatic ductal adenocarcinoma.  |  Rezagholizadeh, F., et al. 2024. Front Immunol. 15: 1283364. PMID: 38357542
7. Spatial interaction and functional status of CD68+SHP2+ macrophages in tumor microenvironment correlate with overall survival of NSCLC.  |  Liu, X., et al. 2024. Front Immunol. 15: 1396719. PMID: 38799432
8. SPI1+CD68+ macrophages as a biomarker for gastric cancer metastasis: a rationale for combined antiangiogenic and immunotherapy strategies.  |  Deng, G., et al. 2024. J Immunother Cancer. 12: PMID: 39455096
9. The 94- to 97-kDa mouse macrophage membrane protein that recognizes oxidized low density lipoprotein and phosphatidylserine-rich liposomes is identical to macrosialin, the mouse homologue of human CD68.  |  Ramprasad, MP., et al. 1995. Proc Natl Acad Sci U S A. 92: 9580-4. PMID: 7568176
10. Flow cytometric analysis of intracellular CD68 molecule expression in normal and malignant haemopoiesis.  |  Strobl, H., et al. 1995. Br J Haematol. 90: 774-82. PMID: 7669655
11. Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins.  |  Holness, CL. and Simmons, DL. 1993. Blood. 81: 1607-13. PMID: 7680921
12. Cell surface expression of mouse macrosialin and human CD68 and their role as macrophage receptors for oxidized low density lipoprotein.  |  Ramprasad, MP., et al. 1996. Proc Natl Acad Sci U S A. 93: 14833-8. PMID: 8962141

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD68 Antibody (KP1) has 250 citations in a variety of scientific publications.

Primary antibodies like Anti-CD68 Antibody (KP1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD68 Antibody (KP1) Specifications

Alternate Product Name: CD68

Product Clone Name: KP1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD68

Epitope Species: human

Product Reactivity/Detection: CD68

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 250 citations in various scientific publications

Available Conjugates/Forms for CD68 Antibody (KP1): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, PerCP: Peridinin Chlorophyll Protein Complex