CD45 Antibody (I3/2.3): sc-52491
- CD45 Antibody (I3/2.3) is a rat monoclonal IgG2b, cited in 9 publications, provided at 200 µg/ml
- recommended for detection of CD45 of mouse origin by WB, IP, IF and FCM
- available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
- See CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45 Antibody (I3/2.3). This work is in progress.
CD45 Antibody (I3/2.3) is a rat monoclonal IgG2b antibody engineered to recognize CD45 protein, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45 is a vital transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells in mice, playing a critical role in regulating immune cell signaling and activation. Structurally, CD45 consists of an extracellular domain with multiple isoforms generated through alternative splicing of exons 4, 5, and 6, a single transmembrane region, and a conserved intracellular domain that possesses phosphatase activity. This structural organization is essential for CD45′s function, as the extracellular domain variations allow for differential interactions with other cell surface molecules, thereby modulating signal transduction pathways. Located on the cell membrane, CD45 dephosphorylates key tyrosine residues on Src family kinases such as Lck and Fyn, as well as on Syk family kinases like ZAP-70, which are crucial for T and B cell receptor signaling. Anti-CD45 antibody (I3/2.3) is raised against T lymphocytes of human origin and is effective in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD45 monoclonal antibody (I3/2.3) recognizes multiple isoforms of CD45, enabling comprehensive analysis of different immune cell subsets and facilitating detailed studies of immune cell differentiation and function. Anti-CD45 antibody (I3/2.3) supports advanced research and diagnostic efforts in immunology and hematology, enhancing our understanding of immune system dynamics and aiding in the development of targeted therapies for immune-related diseases.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD45 Antibody (I3/2.3) | sc-52491 | 200 µg/ml | $322.00 | |||
CD45 Antibody (I3/2.3) FITC | sc-52491 FITC | 200 µg/ml | $336.00 | |||
CD45 Antibody (I3/2.3) PE | sc-52491 PE | 200 µg/ml | $349.00 |