CD42a Double Nickase Plasmid (h): sc-405500-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD42a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD42a Double Nickase Plasmid (h) and CD42a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GP9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD42a Antibody (A-9): sc-166420
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD42a Double Nickase Plasmid (h) | sc-405500-NIC | 20 µg | $410.00 | |||
CD42a Double Nickase Plasmid (h2) | sc-405500-NIC-2 | 20 µg | $410.00 |
GP9 encodes the platelet glycoprotein CD42a, a core component of the GPIb-IX-V receptor complex that mediates platelet adhesion to von Willebrand factor under high shear stress. Through this complex, CD42a supports early hemostatic plug formation and links extracellular ligand binding to intracellular signaling events that regulate cytoskeletal remodeling, platelet activation, and thrombus stabilization. Disruption of GP9 perturbs surface expression and function of the GPIb-IX-V complex, altering platelet adhesion dynamics and downstream activation pathways. Genetic defects in GP9 are associated with inherited macrothrombocytopenia and bleeding phenotypes consistent with Bernard–Soulier syndrome, making GP9 a useful locus for studying platelet biogenesis and adhesion signaling.
CD42a Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GP9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GP9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GP9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GP9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.