CD3-zeta Antibody (D-4): sc-374281

CD3-ζ Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD3-ζ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CD3-ζ (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD3-ζ protein plays a crucial role in T cell activation, as CD3-ζ is part of the T cell receptor (TCR) complex that transmits signals upon recognition of foreign antigens. This signaling is essential for the immune response, as CD3-ζ leads to the activation of T cells, which are vital for adaptive immunity. The CD3 complex, which includes CD3-ζ, consists of five invariant polypeptide chains that form three distinct dimers, facilitating the transmission of activation signals. The ζ chain contains three immunoreceptor tyrosine-based activation motifs (ITAMs), which are critical for recruiting protein kinases like ZAP-70 and Syk, thereby amplifying the signaling cascade that results in T cell activation. The structural integrity and phosphorylation state of these motifs are vital for effective immune responses, making CD3-ζ a key player in immunological signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD3-zeta Antibody (D-4) References:

1. Shed membrane fragment modulation of CD3-zeta during pregnancy: link with induction of apoptosis.  |  Gercel-Taylor, C., et al. 2002. J Reprod Immunol. 56: 29-44. PMID: 12106882
2. T cell receptor complexes containing Fc epsilon RI gamma homodimers in lieu of CD3 zeta and CD3 eta components: a novel isoform expressed on large granular lymphocytes.  |  Koyasu, S., et al. 1992. J Exp Med. 175: 203-9. PMID: 1530959
3. Differential Src family kinase activity requirements for CD3 zeta phosphorylation/ZAP70 recruitment and CD3 epsilon phosphorylation.  |  Lysechko, TL. and Ostergaard, HL. 2005. J Immunol. 174: 7807-14. PMID: 15944285
4. Genetic reconstitution of the T cell receptor (TcR) alpha/beta heterodimer restores the association of CD3 zeta 2 with the TcR/CD3 complex.  |  Blumberg, RS., et al. 1991. Eur J Immunol. 21: 473-81. PMID: 1825637
5. The CD3 zeta subunit contains a phosphoinositide-binding motif that is required for the stable accumulation of TCR-CD3 complex at the immunological synapse.  |  DeFord-Watts, LM., et al. 2011. J Immunol. 186: 6839-47. PMID: 21543646
6. The CD3-zeta chimeric antigen receptor overcomes TCR Hypo-responsiveness of human terminal late-stage T cells.  |  Rappl, G., et al. 2012. PLoS One. 7: e30713. PMID: 22292024
7. T cell CD3ζ deficiency enables multiorgan tissue inflammation.  |  Deng, GM., et al. 2013. J Immunol. 191: 3563-7. PMID: 23980209
8. TCR-CD3ζ gene polymorphisms and expression profile in rheumatoid arthritis.  |  Li, P., et al. 2016. Autoimmunity. 49: 466-471. PMID: 27118209
9. TCR extracellular domain genetically linked to CD28, 2B4/41BB and DAP10/CD3ζ -engineered NK cells mediates antitumor effects.  |  Li, S., et al. 2023. Cancer Immunol Immunother. 72: 769-774. PMID: 35988132
10. Degradation of T cell receptor (TCR)-CD3-zeta complexes after antigenic stimulation.  |  Valitutti, S., et al. 1997. J Exp Med. 185: 1859-64. PMID: 9151711

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD3-ζ Antibody (D-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CD3-ζ Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD3-ζ Antibody (D-4) Specifications

Alternate Product Name: CD247

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-163

Epitope Target: CD3-ζ

Epitope Species: human

Product Reactivity/Detection: CD3-ζ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CD3-ζ Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD3-ζ antibody is CD3-ζ Antibody (6B10.2): sc-1239.