CD10 Double Nickase Plasmid (h): sc-400856-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD10 Double Nickase Plasmid (h) and CD10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MME. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD10 Antibody (F-4): sc-46656
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD10 Double Nickase Plasmid (h) | sc-400856-NIC | 20 µg | $410.00 |
MME encodes CD10 (neprilysin), a zinc-dependent membrane metalloendopeptidase that regulates the extracellular peptide milieu by cleaving diverse bioactive substrates, including enkephalins, natriuretic peptides, and endothelin-related peptides. By modulating ligand availability and receptor signaling amplitude, CD10 influences pathways linked to cell–cell communication, inflammatory tone, and tissue remodeling in epithelial and hematopoietic compartments. CD10 is widely used as a lineage and differentiation marker and its expression state is frequently interrogated in studies of developmental programs and tumor-associated phenotypes, where altered proteolytic processing can reshape microenvironmental signaling. Functional perturbation of MME provides a tractable approach to dissect peptide metabolism networks and downstream transcriptional responses in human cell models.
CD10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MME locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MME. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MME function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MME-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.