Anticorps c-Fos (D-1) recommandé pour la détection de c-Fos d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps c-Fos (D-1): sc-8047

Anticorps c-Fos (D-1) est évalué 3.9 de 5 de 18.
  • y_2020, m_9, d_30, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_18
  • loc_fr_FR, sid_8047, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 121ms
  • REVIEWS, PRODUCT
5
11
4
2
3
0
2
2
1
3

Fiches techniques

    • L'Anticorps c-Fos (D-1) est un monoclonal de souris IgG2b (chaîne légère kappa) fourni en 200 µg/ml
    • dirigé contre les acides aminés 210-335 situés en C-terminus de c-Fos de human
    • Anti-L'Anticorps c-Fos (D-1) est recommandé pour la détection de c-Fos d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
    • L'Anticorps c-Fos (D-1) est disponible conjugué à l'Alexa Fluor® 546 ou Alexa Fluor® 594 pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Réactif TransCruz pour une utilisation en Gel Supershift et ChIP (sc-8047 X, 200 µg/0.1 ml)
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de c-Fos (D-1): sc-8047.

what is the best replacement for Sc-52

Posée par: ismaila
Thank you for your question. This is one of the most popular replacement antibodies for that now discontinued polyclonal antibody, sc-52. However, we offer several different options. Please contact our Technical Service Department for a recommendation based on your intended research application(s) and species reactivity.
Répondue par: Technical Support
Date de publication: 2019-05-25

I used immunofluorescence staining in the hypothalamus of rat, and as a result c-fos was expressed in the cytoplasm. It's not expressed in the nucleus. Is it correct? 0 answers

Posée par: Emma Li
Thank you for your question. According to the Human Protein Atlas, c-Fos (FOS) is localized to the nucleoplasm. Nuclear and cytoplasmic expression may be detected in most tissues. https://www.proteinatlas.org/ENSG00000170345-FOS/pathology
Répondue par: Technical Support
Date de publication: 2019-05-25

Can this antibody be used for the detection of c-fos in the macaque monkey. If not, which of your antibodies would you recommend?

Posée par: myprefrontal
Thank you for your question. We have not tested this antibody for reactivity with macaque, and are not aware of any publications citing use of the antibody in that species. We do offer an alternative antibody, c-Fos (6-2H-2F): sc-447, that has been published at least twice for detection of c-Fos in monkey. Please see PMID's: 9108029 and 11937718 for those references. A link to that product page on our website is below. https://www.scbt.com/scbt/product/c-fos-antibody-6-2h-2f
Répondue par: Technical Support
Date de publication: 2017-11-01

Can I still buy monoclonal antibodies from this company?

Posée par: KarenLindquist
Thank you for your question! Yes, you can certainly buy monoclonal antibodies from us! Please contact us at any time and we'd be glad to help you! You can reach us by phone at 800-457-3801 or by email at [email protected] or by live chat directly on our website, www.scbt.com.
Répondue par: Tech Service
Date de publication: 2017-05-29

Could you please advise if this antibody is able to detect Fra proteins as is the case with the discontinued sc 253.

Posée par: Conor
Thank you for your question. The equivalent replacement mouse monoclonal antibodies to sc-253 are sc-166940: c-Fos (E-8) and sc-271243: c-Fos (C-10). Both of these mouse monoclonal antibodies are raised against the same peptide antigen used to generate the polyclonal antibody, sc-253. These recommended replacement mouse monoclonal antibodies are also recommended for detection of c-Fos, Fos B, Fra-1 and Fra-2 of mouse, rat, human, zebrafish and avian origin. Links to these products are provided below for your reference. https://www.scbt.com/scbt/product/c-fos-antibody-e-8 https://www.scbt.com/scbt/product/c-fos-antibody-c-10
Répondue par: Technical Support
Date de publication: 2017-01-19

I want to detect c-fos in my porcine samples. Will this antibody work?

Posée par: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Répondue par: TechService7
Date de publication: 2016-12-23
  • y_2020, m_9, d_30, h_15CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_6
  • loc_fr_FR, sid_8047, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 123ms
  • QUESTIONS, PRODUCT
Rated 5 de 5 de par - We examined the effects of a 3-week treatment of olanzapine (Olz) on c-Fos protein expression in stress-relevant brain sub/ regions, its relationship with isolation-induced behavioral changes, and potential sites of Olz action on control and male rats exposed to 6 weeks of chronic social isolation an animal model of depression.
Date de publication: 2018-12-07
Rated 5 de 5 de par 功能很齐全也很棒 我两个月前购买了这个产品,用它来做免疫荧光看的非常清楚,我想再买一盒接着做,同时我最满意的是它的性价比,很实惠也很实用,值得购买
Date de publication: 2018-05-21
Rated 2 de 5 de par It does not work well It did not work as well as other company's ab when I tested it by 1: 500.
Date de publication: 2018-04-03
Rated 2 de 5 de par No nuclear staining Level of expression appears comparable to other fos antibodies, but does not stain the the nucleus and non-specific binding is present at high concentrations.
Date de publication: 2018-03-14
Rated 1 de 5 de par Not satisfied! I tried a free sample of this antibody on free floating mouse brain sections and it didn't work. I used a concentration of 1:50 for the primary antibody, and 1:300 for secondary antibody (anti-mouse 488) and I didn't got any signal. Really not satisfied.
Date de publication: 2018-02-23
Rated 5 de 5 de par 功能很出色 我上一个月染得的fos,很出色,背景很清晰,很清晰。我上一个月染得的fos,很出色,背景很清晰,很清晰。
Date de publication: 2018-02-05
Rated 1 de 5 de par Disappointed I tried a sample of this antibody on my free-floating mouse brain sections which I have another set stained with my old, running out antibody to compare. It did not work at all at concentration ranging 1:100-2000.
Date de publication: 2017-11-27
Rated 1 de 5 de par Did not label cFos cells in sheep hypothalamus Using free-floating sections from sheep tissue, and an immunoperoxidase method of IHC, we did not see any immunostained cells using this antibody at dilutions of 1: 500, 1:1000, 1:2000.
Date de publication: 2017-11-08
  • y_2020, m_9, d_30, h_15
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_18
  • loc_fr_FR, sid_8047, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 21ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. est leader mondial dans le développement de produits pour la recherche biomédicale. Contactez-nous au 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. Tous Droits Réservés. "Santa Cruz Biotechnology", et le logo Santa Cruz Biotechnology, Inc., "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", le logo San Juan Ranch, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", et "EZ Touch" sont des marques déposées de Santa Cruz Biotechnology, Inc.
Toutes les marques sont la propriété de leurs propriétaires respectifs.