Brn-2/BRN2/POU3F2 Antibody (ZD-12): sc-81981
- Brn-2/BRN2/POU3F2 Antibody (ZD-12) is a mouse monoclonal IgG2b κ provided at 100 µg/ml
- raised against recombinant Brn-2 of human origin
- recommended for detection of precursor and mature Brn-2 of mouse, rat and human origin by WB, IP and ELISA
- See Brn-2/BRN2/POU3F2 (B-2): sc-393324 for Brn-2/BRN2/POU3F2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Brn-2/BRN2/POU3F2 Antibody (ZD-12). This work is in progress.
Brn-2 Antibody (ZD-12) is a mouse monoclonal IgG2b antibody that detects Brn-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Brn-2 is a member of the Brn family of transcription factors, specifically classified as a class III POU domain protein, and plays a pivotal role in the early embryonic development of the central nervous system. Brn-2 is predominantly expressed during the development of the forebrain and is coexpressed in most layer II-V cortical neurons, where Brn-2 is essential for regulating the initiation of radial migration of cortical neurons. This process is crucial for proper brain architecture and function, as Brn-2 ensures that neurons migrate to their appropriate locations during development. Additionally, Brn-2 is implicated in the differentiation of smooth muscle cells, highlighting Brn-2′s importance beyond neuronal development. Brn-2′s ability to interact with other transcription factors and regulatory proteins further underscores its significance in gene expression modulation, making anti-Brn-2 antibody (ZD-12) an invaluable tool for researchers studying neural development and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Brn-2/BRN2/POU3F2 Antibody (ZD-12) References:
- Domains of Brn-2 that mediate homodimerization and interaction with general and melanocytic transcription factors. | Smit, DJ., et al. 2000. Eur J Biochem. 267: 6413-22. PMID: 11029584
- The POU proteins Brn-2 and Oct-6 share important functions in Schwann cell development. | Jaegle, M., et al. 2003. Genes Dev. 17: 1380-91. PMID: 12782656
- Brn-2 expression controls melanoma proliferation and is directly regulated by beta-catenin. | Goodall, J., et al. 2004. Mol Cell Biol. 24: 2915-22. PMID: 15024079
- The Brn-2 transcription factor links activated BRAF to melanoma proliferation. | Goodall, J., et al. 2004. Mol Cell Biol. 24: 2923-31. PMID: 15024080
- The neuronal POU transcription factor Brn-2 interacts with Jab1, a gene involved in the onset of neurodegenerative diseases. | Huang, YT., et al. Neurosci Lett. 382: 175-8. PMID: 15911144
- Brn-2 represses microphthalmia-associated transcription factor expression and marks a distinct subpopulation of microphthalmia-associated transcription factor-negative melanoma cells. | Goodall, J., et al. 2008. Cancer Res. 68: 7788-94. PMID: 18829533
- Mutant huntingtin fragment selectively suppresses Brn-2 POU domain transcription factor to mediate hypothalamic cell dysfunction. | Yamanaka, T., et al. 2010. Hum Mol Genet. 19: 2099-112. PMID: 20185558
- A phosphatidylinositol 3-kinase-Pax3 axis regulates Brn-2 expression in melanoma. | Bonvin, E., et al. 2012. Mol Cell Biol. 32: 4674-83. PMID: 22988297
- Integrin α3β1 Promotes Invasive and Metastatic Properties of Breast Cancer Cells through Induction of the Brn-2 Transcription Factor. | Miskin, RP., et al. 2021. Cancers (Basel). 13: PMID: 33513758
- Predominant expression of Brn-2 in the postmitotic neurons of the developing mouse neocortex. | Hagino-Yamagishi, K., et al. 1997. Brain Res. 752: 261-8. PMID: 9106466
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Brn-2/BRN2/POU3F2 Antibody (ZD-12) | sc-81981 | 100 µg/ml | $339.00 |