BRD9 CRISPR/Cas9 KO Plasmid (m): sc-430581
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BRD9 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BRD9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRD9 CRISPR/Cas9 KO Plasmid (m) | sc-430581 | 20 µg | $397.00 |
Overview
Mouse Brd9 encodes BRD9, a bromodomain-containing chromatin reader that recognizes acetyl-lysine marks on histones and contributes to ATP-dependent chromatin remodeling. BRD9 is a defining component of non-canonical BAF (ncBAF/GBAF) complexes and supports enhancer and promoter regulation, transcriptional programs controlling proliferation, lineage specification, and cellular identity. Through these epigenetic functions, BRD9 influences pathways linked to DNA damage responses, cell cycle control, and differentiation state transitions. Dysregulated BRD9-associated chromatin remodeling and transcriptional circuitry has been implicated in contexts relevant to oncogenic dependency and developmental phenotypes, making it a useful node for mechanistic studies in disease models.
BRD9 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Brd9 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Brd9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Brd9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BRD9 protein expression.
This CRISPR knockout system enables efficient generation of Brd9-deficient cell models for investigation of BRD9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Brd9 exon(s) critical for BRD9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Brd9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BRD9 CRISPR/Cas9 KO Plasmid (m) and BRD9 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Brd9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BRD9 HDR Plasmid (m) and BRD9 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Brd9 homology arms to support homology-directed repair at defined Brd9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.