Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BRD3 CRISPR/Cas9 KO Plasmid (m): sc-426528
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BRD3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BRD3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BRD3 Antibody (2088C3a): sc-81202
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BRD3 CRISPR/Cas9 KO Plasmid (m) | sc-426528 | 20 µg | $397.00 |
Overview
Brd3 encodes BRD3, a member of the BET family of bromodomain-containing proteins that bind acetylated lysines on histones to interpret chromatin marks and coordinate transcriptional programs. In the nucleus, BRD3 contributes to RNA polymerase II–dependent gene regulation, enhancer activity, and cell-cycle–linked transcriptional control through interactions with acetylated chromatin and transcriptional co-regulators. These functions position BRD3 within epigenetic signaling pathways that influence differentiation, proliferation, and stress-responsive gene expression. Dysregulated BET protein activity, including BRD3-associated transcriptional networks, has been implicated in oncogenic gene expression states and inflammatory signaling contexts, supporting its relevance in mechanistic disease modeling.
BRD3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Brd3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Brd3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Brd3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BRD3 protein expression.
This CRISPR knockout system enables efficient generation of Brd3-deficient cell models for investigation of BRD3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Brd3 exon(s) critical for BRD3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Brd3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BRD3 CRISPR/Cas9 KO Plasmid (m) and BRD3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Brd3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BRD3 HDR Plasmid (m) and BRD3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Brd3 homology arms to support homology-directed repair at defined Brd3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.