BPTF Double Nickase Plasmid (h): sc-404092-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BPTF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BPTF Double Nickase Plasmid (h) and BPTF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BPTF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BPTF Antibody (2343C3a): sc-81088
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BPTF Double Nickase Plasmid (h) | sc-404092-NIC | 20 µg | $410.00 | |||
BPTF Double Nickase Plasmid (h2) | sc-404092-NIC-2 | 20 µg | $410.00 |
BPTF (bromodomain PHD finger transcription factor) is a core subunit of the NURF chromatin remodeling complex that couples recognition of histone modifications to ATP-dependent nucleosome sliding, thereby shaping chromatin accessibility and transcriptional programs. Through its PHD finger and bromodomain, BPTF integrates signals from H3K4 methylation and histone acetylation to regulate gene expression networks controlling development, lineage specification, and cell-cycle progression. BPTF-dependent remodeling influences enhancer activity and transcription factor occupancy, linking epigenetic state to RNA polymerase II–mediated transcription. Dysregulated BPTF expression or function has been associated with altered chromatin states and transcriptional dependencies observed across multiple cancer and neurodevelopment-related contexts, supporting its use as a research target in epigenetics and transcription biology.
BPTF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BPTF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BPTF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BPTF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BPTF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.