Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BDP1 CRISPR/Cas9 KO Plasmid (m): sc-422508
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BDP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BDP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BDP1 Antibody (B-6): sc-515058
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BDP1 CRISPR/Cas9 KO Plasmid (m) | sc-422508 | 20 µg | $397.00 |
Overview
Ptpn18 encodes a protein tyrosine phosphatase implicated in regulating receptor tyrosine kinase signaling and downstream phosphorylation-dependent pathways that shape cell growth, adhesion, and motility. In mouse cells, PTPN18 has been linked to modulation of EGFR/ErbB-family signaling and protein trafficking processes that influence signal duration and spatial organization. Through dephosphorylation of specific substrates, PTPN18 can impact MAPK and PI3K-associated outputs, integrating cues that affect cytoskeletal dynamics and cellular stress responses. Dysregulated phosphatase activity and altered RTK signaling are widely associated with oncogenic signaling programs and inflammatory phenotypes, making Ptpn18 a useful target for mechanistic studies in disease-relevant models.
BDP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ptpn18 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ptpn18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ptpn18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BDP1 protein expression.
This CRISPR knockout system enables efficient generation of Ptpn18-deficient cell models for investigation of BDP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ptpn18 exon(s) critical for BDP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ptpn18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BDP1 CRISPR/Cas9 KO Plasmid (m) and BDP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ptpn18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BDP1 HDR Plasmid (m) and BDP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ptpn18 homology arms to support homology-directed repair at defined Ptpn18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.