Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BAT1 CRISPR/Cas9 KO Plasmid (h): sc-403785
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BAT1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BAT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAT1 CRISPR/Cas9 KO Plasmid (h) | sc-403785 | 20 µg | $397.00 |
Overview
DDX39B encodes BAT1 (also known as UAP56), a conserved DEAD-box RNA helicase that supports ATP-dependent remodeling of RNA–protein complexes during pre-mRNA splicing and mRNA export. BAT1 functions with the TREX/export machinery to couple transcription, spliceosome dynamics, and nuclear export, thereby shaping gene expression programs and RNA quality control. Through these roles, DDX39B activity influences cellular proliferation, stress responses, and innate immune signaling linked to RNA processing. Altered regulation of RNA helicases and mRNP biogenesis is frequently associated with oncogenic transcriptional states and inflammatory phenotypes, making BAT1 a useful target for mechanistic studies of RNA metabolism in disease-relevant models.
BAT1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DDX39B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DDX39B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DDX39B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BAT1 protein expression.
This CRISPR knockout system enables efficient generation of DDX39B-deficient cell models for investigation of BAT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DDX39B exon(s) critical for BAT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DDX39B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BAT1 CRISPR/Cas9 KO Plasmid (h) and BAT1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DDX39B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BAT1 HDR Plasmid (h) and BAT1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DDX39B homology arms to support homology-directed repair at defined DDX39B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.