B23/Nucleophosmin Antibody (3F291): sc-70392

B23 Antibody (3F291) is a mouse monoclonal IgG1 antibody that detects B23 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. B23, also known as nucleophosmin, plays a crucial role in ribosomal assembly and the transport of ribosomal RNA, making B23 essential for protein synthesis and cellular function. B23 is predominantly located in the nucleolus, where B23 is involved in the maturation of ribosomal subunits, and proper localization is vital for maintaining efficient ribosome biogenesis. Additionally, B23 is highly phosphorylated by Cdc2 kinase during mitosis, which is important for regulating B23 function and interactions during cell division. Anti-B23 antibody (3F291) is an invaluable tool for researchers studying nucleolar dynamics, ribosome assembly, and the intricate processes of cellular proliferation, providing insights into the fundamental mechanisms that govern cell growth and development.

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B23/Nucleophosmin Antibody (3F291) References:

1. In vivo interaction of nucleophosmin/B23 and protein C23 during cell cycle progression in HeLa cells.  |  Liu, HT. and Yung, BY. 1999. Cancer Lett. 144: 45-54. PMID: 10503877
2. The carboxyl terminal sequence of nucleolar protein B23.1 is important in its DNA polymerase alpha-stimulatory activity.  |  Umekawa, H., et al. 2001. J Biochem. 130: 199-205. PMID: 11481036
3. The RNA binding activity of a ribosome biogenesis factor, nucleophosmin/B23, is modulated by phosphorylation with a cell cycle-dependent kinase and by association with its subtype.  |  Okuwaki, M., et al. 2002. Mol Biol Cell. 13: 2016-30. PMID: 12058066
4. UV stimulation of nucleophosmin/B23 expression is an immediate-early gene response induced by damaged DNA.  |  Wu, MH. and Yung, BY. 2002. J Biol Chem. 277: 48234-40. PMID: 12374805
5. Involvement of nucleophosmin/B23 in TPA-induced megakaryocytic differentiation of K562 cells.  |  Hsu, CY. and Yung, BY. 2003. Br J Cancer. 89: 1320-6. PMID: 14520467
6. Protein B23 (M.W./pI = 37 kD/5.1) is the only major protein extracted from HeLa nucleoli with 3M urea.  |  Yung, BY., et al. 1992. Life Sci. 51: 915-20. PMID: 1518368
7. Essential role of the B23/NPM core domain in regulating ARF binding and B23 stability.  |  Enomoto, T., et al. 2006. J Biol Chem. 281: 18463-72. PMID: 16679321
8. Distribution of nucleolar proteins B23 and nucleolin during mouse spermatogenesis.  |  Biggiogera, M., et al. 1991. Chromosoma. 100: 162-72. PMID: 1710179
9. Translocation of nucleolar phosphoprotein B23 (37 kDa/pI 5.1) induced by selective inhibitors of ribosome synthesis.  |  Yung, BY., et al. 1985. Biochim Biophys Acta. 826: 167-73. PMID: 3907710
10. Cell cycle-dependent translocations of a major nucleolar phosphoprotein, B23, and some characteristics of its variants.  |  Zatsepina, OV., et al. 1997. Eur J Cell Biol. 73: 58-70. PMID: 9174672

The preferred B23/Nucleophosmin antibody is B23/Nucleophosmin Antibody (0412): sc-47725.