Date published: 2025-9-9

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Anticorps B23/Nucleophosmin (3F291): sc-70392

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Fiches techniques
  • L'Anticorps B23/Nucleophosmin (3F291) est un monoclonal de souris IgG1 κ, cité dans 9 publications, fourni en 200 µg/ml
  • élevé contre une protéine recombinante correspondant à la N-terminus de B23 d'origine human.
  • recommandé pour la détection de B23 d'origine mouse, rat et human par WB, IP, IF et IHC(P)
  • Voir B23/Nucleophosmin (E-3): sc-271737 pour d'autres anticorps conjugués B23/Nucleophosmin, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • m-IgG Fc BP-HRP et m-IgG1 BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps B23/Nucleophosmin (3F291) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps B23/Nucleophosmin (3F291) (voir Informations pour la commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

The transport of proteins across the nuclear envelope is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex, and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. For example, two cytosolic factors critically involved in the recognition and docking process are the karyopherin α and karyopherin β proteins. The karyopherin holoenzyme is a heterodimer of α and β subunits. The nuclear protein B23 (also referred to as nucleophosmin) is involved in ribosomal assembly and rRNA transport. B23 is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps B23/Nucleophosmin (3F291)

sc-70392
200 µg/ml
$316.00

B23/Nucleophosmin (3F291): m-IgG Fc BP-HRP Kit

sc-539270
200 µg Ab; 10 µg BP
$354.00

B23/Nucleophosmin (3F291): m-IgG1 BP-HRP Kit

sc-541368
200 µg Ab; 20 µg BP
$354.00

What is the recommended starting dilution for Western blot for sc-70392: B23 (3F291) monoclonal antibody?

Posée par: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Répondue par: Technical Support
Date de publication: 2017-03-22
  • y_2025, m_8, d_31, h_8CST
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Rated 1 de 5 de par Does not work for IF in mouse neruonsTested free sample on ICC with dilution of 1:25 and 1:100 in primary neurons from mouse cortex. Does not work at all for ICC
Date de publication: 2018-12-05
Rated 4 de 5 de par Positive result in WB in human B23 transfectedPositive result in WB in human B23 transfected 293T and HEL 92.1.7 whole cell lysates. -SCBT QC
Date de publication: 2015-05-05
Rated 4 de 5 de par Good specificity in WB in HeLa whole cell lysateGood specificity in WB in HeLa whole cell lysate. -SCBT QC
Date de publication: 2015-01-14
Rated 4 de 5 de par Published data for IP in HEK cellsPublished data for IP in HEK cells. -SCBT Publication Review
Date de publication: 2015-01-11
Rated 4 de 5 de par Band of interest observed in WB in humanBand of interest observed in WB in human B23 transfected 293 whole cell lysate. -SCBT QC
Date de publication: 2013-01-12
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Anticorps B23/Nucleophosmin (3F291) est évalué 3.4 de 5 de 5.
  • y_2025, m_8, d_31, h_8
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