Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ATP7B CRISPR/Cas9 KO Plasmid (m): sc-419260
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATP7B CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ATP7B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATP7B Antibody (A-11): sc-373964
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP7B CRISPR/Cas9 KO Plasmid (m) | sc-419260 | 20 µg | $397.00 |
Overview
Mouse Atp7b encodes ATP7B, a P-type ATPase copper transporter that regulates intracellular copper distribution by shuttling between the trans-Golgi network and vesicular compartments in response to copper load. ATP7B supports cuproenzyme maturation in the secretory pathway and mediates copper sequestration and export, integrating with cellular metal homeostasis and oxidative stress responses. Disruption of ATP7B-dependent copper handling perturbs redox balance, protein trafficking, and mitochondrial function, making Atp7b a key node for studying copper metabolism and related pathophysiology. In mice, Atp7b is widely used to model genetic copper overload phenotypes and to interrogate hepatic and neurologic mechanisms linked to metal dyshomeostasis.
ATP7B CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Atp7b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Atp7b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Atp7b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ATP7B protein expression.
This CRISPR knockout system enables efficient generation of Atp7b-deficient cell models for investigation of ATP7B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Atp7b exon(s) critical for ATP7B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Atp7b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ATP7B CRISPR/Cas9 KO Plasmid (m) and ATP7B CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Atp7b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ATP7B HDR Plasmid (m) and ATP7B HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Atp7b homology arms to support homology-directed repair at defined Atp7b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.