Angiotensin Receptor/AT1/AGTR1 Antibody (G-3): sc-515884

AT1 Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects AT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AT1 (G-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AT1 protein, also known as Angiotensin II type-1 receptor, plays a crucial role in blood pressure and fluid balance regulation by mediating Angiotensin II′s physiological effects, including vasoconstriction, aldosterone release stimulation, and sodium reabsorption enhancement. AT1 protein activates multiple intracellular signaling pathways contributing to cardiovascular homeostasis. As a G protein-coupled receptor family member, AT1 features seven transmembrane domains that facilitate extracellular signal transmission into cells. Ang II binding triggers conformational changes in AT1 protein, activating downstream signaling cascades, including phospholipase C gamma pathway, leading to phosphorylation of Shc and MAP kinases. This activation modulates various cellular responses, including growth factor signaling and cellular proliferation. AT1 protein′s interaction with Src kinases and multiple phosphorylation sites highlights AT1′s significance in cardiovascular signaling and potential therapeutic targeting for hypertension and heart failure.

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Angiotensin Receptor/AT1/AGTR1 Antibody (G-3) References:

1. Renal responses to AT1 receptor blockade.  |  Navar, LG., et al. 2000. Am J Hypertens. 13: 45S-54S. PMID: 10678288
2. Pharmacology of AT1-receptor blockers.  |  Unger, T. 2001. Blood Press Suppl. 5-10. PMID: 11683476
3. Multiple actions of angiotensin II in hypertension: benefits of AT1 receptor blockade.  |  Schiffrin, EL. and Touyz, RM. 2003. J Am Coll Cardiol. 42: 911-3. PMID: 12957442
4. AT1 and AT2 receptor in the kidney: role in health and disease.  |  Siragy, HM. 2004. Semin Nephrol. 24: 93-100. PMID: 15017521
5. Isolation of a cDNA encoding the vascular type-1 angiotensin II receptor.  |  Murphy, TJ., et al. 1991. Nature. 351: 233-6. PMID: 2041570
6. Vasoconstrictor-induced protein-tyrosine phosphorylation in cultured vascular smooth muscle cells.  |  Tsuda, T., et al. 1991. FEBS Lett. 285: 44-8. PMID: 2065781
7. AT1 Receptor as a Potential Target in Amphetamine-induced Neuroinflammation.  |  Cabrera, RJ., et al. 2022. Protein Pept Lett. 29: 371-374. PMID: 35362369
8. Direct stimulation of Jak/STAT pathway by the angiotensin II AT1 receptor.  |  Marrero, MB., et al. 1995. Nature. 375: 247-50. PMID: 7746328
9. Angiotensin II-induced protein tyrosine phosphorylation in neonatal rat cardiac fibroblasts.  |  Schorb, W., et al. 1994. J Biol Chem. 269: 19626-32. PMID: 8034731
10. Angiotensin II stimulates tyrosine phosphorylation of phospholipase C-gamma 1 in vascular smooth muscle cells.  |  Marrero, MB., et al. 1994. J Biol Chem. 269: 10935-9. PMID: 8144678
11. Angiotensin II induces 3CH134, a protein-tyrosine phosphatase, in vascular smooth muscle cells.  |  Duff, JL., et al. 1993. J Biol Chem. 268: 26037-40. PMID: 8253712
12. Angiotensin II receptors and angiotensin II receptor antagonists.  |  Timmermans, PB., et al. 1993. Pharmacol Rev. 45: 205-51. PMID: 8372104

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Angiotensin Receptor/AT1/AGTR1 Antibody (G-3) has 40 citations in a variety of scientific publications.

Primary antibodies like Anti-Angiotensin Receptor/AT1/AGTR1 Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Angiotensin Receptor/AT1/AGTR1 Antibody (G-3) Specifications

Alternate Product Name: AT1

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 313-337

Epitope Target: AT₁

Epitope Species: human

Product Reactivity/Detection: AT₁ types 1A and 1B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 40 citations in various scientific publications

Available Conjugates/Forms for Angiotensin Receptor/AT1/AGTR1 Antibody (G-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin