Arc Double Nickase Plasmid (h): sc-400231-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Arc Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Arc Double Nickase Plasmid (h) and Arc Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ARC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Arc Antibody (C-7): sc-17839
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Arc Double Nickase Plasmid (h) | sc-400231-NIC | 20 µg | $410.00 | |||
Arc Double Nickase Plasmid (h2) | sc-400231-NIC-2 | 20 µg | $410.00 |
ARC encodes activity-regulated cytoskeleton-associated protein (Arc), an immediate early gene product that couples neuronal activity to synaptic remodeling. Arc regulates AMPA receptor endocytosis, actin cytoskeleton dynamics, and trafficking of postsynaptic components, thereby shaping long-term potentiation/depression and homeostatic synaptic scaling. Through interactions with endocytic and cytoskeletal machinery, Arc integrates signaling downstream of glutamatergic neurotransmission and calcium-dependent pathways to modulate experience-dependent plasticity. Altered ARC expression or function has been linked to mechanisms implicated in neuropsychiatric and neurodegenerative research, including dysregulated synaptic connectivity and memory-associated processes.
Arc Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ARC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ARC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ARC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ARC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.