Amylase Antibody (C-12): sc-514313

Amylase Antibody (C-12) is a mouse monoclonal IgG3 kappa light chain antibody specifically engineered to detect both precursor and mature forms of Amylase 1 (salivary) across mouse, rat, and human species. Amylase monoclonal antibody (C-12) targets an epitope mapped to amino acids 361-384 near the C-terminus of human amylase, also known as alpha-amylase, ensuring precise and reliable binding. Alpha-amylase plays a vital role in carbohydrate metabolism by catalyzing the breakdown of starches into simpler sugars, which is essential for efficient energy production and digestive processes. Post-translational modifications, such as glycosylation, are crucial for proper folding, stability, and secretion of amylase, affecting enzymatic activity and interactions within the cell. Understanding these modifications is important for studying metabolic regulation, pancreatic function, and related disorders, making anti-Amylase antibody (C-12) an invaluable tool for researchers. This product is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), providing versatility across various experimental applications. Available in both non-conjugated form and multiple conjugated variants, including horseradish peroxidase (HRP) and Alexa Fluor® conjugates, anti-Amylase antibody (C-12) accommodates a wide range of detection needs. In humans, different haplotypes of amylase influence the number and types of salivary and pancreatic isoforms, while in mice, tissue-specific expression underscores physiological significance. By enabling detailed analysis of amylase′s post-translational modifications and function, Amylase monoclonal antibody (C-12) supports advanced research into enzyme regulation, metabolic pathways, and disease mechanisms associated with amylase dysregulation.

The preferred Amylase antibody is Amylase Antibody (G-10): sc-46657.