Anticorps Akt3 (EE-M14) recommandé pour la détection de Akt3 d'origine mouse, rat et human par WB, IP et ELISA

Anticorps Akt3 (EE-M14): sc-134254

Anticorps Akt3 (EE-M14) est évalué 4.6 de 5 de 9.
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Fiches techniques

    • L'Anticorps Akt3 (EE-M14) est un monoclonal de souris IgG2a κ, cité dans 6 publications, fourni en 100 µg/ml
    • raised against recombinant Akt3 protein of human origin
    • recommandé pour la détection de Akt3 d'origine mouse, rat et human par WB, IP et ELISA
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Akt3 (EE-M14): sc-134254.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Akt3 Antibody (EE-M14). This work is in progress.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with Akt3 (EE-M14): sc-134254 antibody?

Posée par: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Répondue par: Technical Support
Date de publication: 2017-03-27
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Rated 4 de 5 de par Good for WB I've tested this antibody in western blotting and it worked well at 1:1000 dilution.
Date de publication: 2021-01-28
Rated 4 de 5 de par Worked for WB Gave a good band after longer exposure time. Used 1:200 for mouse tissue lysates
Date de publication: 2018-03-23
Rated 4 de 5 de par Good antibody Worked well with western blotting at 1:500 dilution.
Date de publication: 2017-09-27
Rated 4 de 5 de par a little weak this antibody is specific , but the band is a little weak.
Date de publication: 2017-07-07
Rated 5 de 5 de par Works well for Western with HSC-3 cells WB: 1:200. Single strong band with HSC-3 and cal-27 cells.
Date de publication: 2017-01-10
Rated 5 de 5 de par Single strong band in mouse NIH/3T3 samples Single strong band in mouse NIH/3T3 samples. -SCBT QC
Date de publication: 2015-03-24
Rated 5 de 5 de par Works well for Western in MCF7 cells Works well for Western in MCF7 cells. -SCBT QC
Date de publication: 2015-02-26
Rated 5 de 5 de par Great WB data in HeLa cells Great WB data in HeLa cells. -SCBT QC
Date de publication: 2015-02-06
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