Adenylate cyclase 3/AC3/ADCY3 CRISPR/Cas9 KO Plasmid (h2): sc-400407-KO-2

Overview

ADCY3 encodes adenylate cyclase 3 (AC3), a membrane-associated enzyme that converts ATP to cyclic AMP, thereby shaping second-messenger signaling downstream of diverse G protein–coupled receptors. By regulating intracellular cAMP levels, AC3 influences PKA and EPAC-dependent pathways that control transcriptional programs, ion channel activity, and cellular metabolic responses. AC3 is highly studied in sensory and neuroendocrine contexts and contributes to cAMP microdomain signaling that tunes stimulus–response coupling. Genetic and functional evidence links ADCY3-associated signaling to metabolic phenotypes and neurobehavioral traits, making it relevant for mechanistic studies of cAMP-driven regulation in human cells.

Adenylate cyclase 3/AC3/ADCY3 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the ADCY3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ADCY3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ADCY3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Adenylate cyclase 3/AC3/ADCY3 protein expression.

This CRISPR knockout system enables efficient generation of ADCY3-deficient cell models for investigation of Adenylate cyclase 3/AC3/ADCY3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting ADCY3 exon(s) critical for Adenylate cyclase 3/AC3/ADCY3 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple ADCY3 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Adenylate cyclase 3/AC3/ADCY3 CRISPR/Cas9 KO Plasmid (h) and Adenylate cyclase 3/AC3/ADCY3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ADCY3 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Adenylate cyclase 3/AC3/ADCY3 HDR Plasmid (h) and Adenylate cyclase 3/AC3/ADCY3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ADCY3 homology arms to support homology-directed repair at defined ADCY3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.