ABAT Antibody (B-5): sc-393142

ABAT Antibody (B-5) is a mouse monoclonal IgG1 antibody that detects ABAT in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ABAT antibody (B-5) recognizes ABAT, a crucial mitochondrial matrix protein that plays a significant role in the metabolism of gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. By catalyzing the conversion of GABA into succinic semialdehyde, ABAT helps regulate GABA levels, which is vital for maintaining neuronal excitability and preventing excessive neuronal firing. Proper functioning of ABAT is essential, as deficiencies can lead to GABA-AT deficiency, a condition associated with severe neurological symptoms such as hypotonia, hyperreflexia, and refractory seizures. ABAT exists as a homodimer and requires pyridoxal phosphate as a cofactor for enzymatic activity, highlighting the importance of cofactor availability in metabolic processes. ABAT is expressed in various tissues, including the liver, brain, pancreas, kidney, placenta, and heart, indicating widespread physiological relevance. The gene encoding ABAT is located on human chromosome 16p13.2, and understanding ABAT function and interactions is crucial for elucidating the underlying mechanisms of neurological disorders.

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ABAT Antibody (B-5) References:

1. The GABA transaminase, ABAT, is essential for mitochondrial nucleoside metabolism.  |  Besse, A., et al. 2015. Cell Metab. 21: 417-27. PMID: 25738457
2. Prevalence of Pathogenic Copy Number Variation in Adults With Pediatric-Onset Epilepsy and Intellectual Disability.  |  Borlot, F., et al. 2017. JAMA Neurol. 74: 1301-1311. PMID: 28846756
3. Metabolomics Profile in ABAT Deficiency Pre- and Post-treatment.  |  Koenig, MK. and Bonnen, PE. 2019. JIMD Rep. 43: 13-17. PMID: 29480352
4. ABAT and ALDH6A1, regulated by transcription factor HNF4A, suppress tumorigenic capability in clear cell renal cell carcinoma.  |  Lu, J., et al. 2020. J Transl Med. 18: 101. PMID: 32093682
5. Exploring the Genetic Association of the ABAT Gene with Alzheimer's Disease.  |  Zheng, Q., et al. 2021. Mol Neurobiol. 58: 1894-1903. PMID: 33404980
6. ABAT targeted by miR-183-5p regulates cell functions in liver cancer.  |  Han, H., et al. 2021. Int J Biochem Cell Biol. 141: 106116. PMID: 34742920
7. Genetic variations in GABA metabolism and epilepsy.  |  Feng, Y., et al. 2022. Seizure. 101: 22-29. PMID: 35850019
8. ABAT gene expression associated with the sensitivity of hypomethylating agents in myelodysplastic syndrome through CXCR4/mTOR signaling.  |  Zhao, G., et al. 2022. Cell Death Discov. 8: 398. PMID: 36163180
9. Empagliflozin in Patients with Chronic Kidney Disease.  |  ,., et al. 2023. N Engl J Med. 388: 117-127. PMID: 36331190
10. Corrigendum to 'ABAT targeted by miR-183-5p regulates cell functions in liver cancer' [Int. J. Biochem. Cell Biol. 141 (2021) 106116].  |  Han, H., et al. 2023. Int J Biochem Cell Biol. 154: 106342. PMID: 36521342

The preferred ABAT antibody is ABAT Antibody (B-12): sc-393769.