Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ZO-2 CRISPR/Cas9 KO Plasmid (h): sc-401395
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZO-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ZO-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ZO-2 Antibody (E-3): sc-515115
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZO-2 CRISPR/Cas9 KO Plasmid (h) | sc-401395 | 20 µg | $397.00 |
Overview
TJP2 encodes the tight junction scaffold protein ZO-2, a MAGUK family member that links claudins and occludin to the cortical actin cytoskeleton to support epithelial and endothelial barrier integrity. ZO-2 also coordinates junctional assembly with signaling pathways that regulate polarity and proliferation, including interactions influencing Rho GTPase dynamics and junction-associated transcriptional programs. Through its roles in paracellular permeability control and maintenance of apico-basal organization, altered TJP2/ZO-2 function is relevant to studies of barrier dysfunction and tissue homeostasis. Dysregulation of tight junction components such as ZO-2 is frequently investigated in contexts of inflammation, fibrosis, and epithelial transformation where junction remodeling and polarity loss are prominent.
ZO-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TJP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TJP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TJP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ZO-2 protein expression.
This CRISPR knockout system enables efficient generation of TJP2-deficient cell models for investigation of ZO-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TJP2 exon(s) critical for ZO-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TJP2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ZO-2 CRISPR/Cas9 KO Plasmid (h) and ZO-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TJP2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ZO-2 HDR Plasmid (h) and ZO-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TJP2 homology arms to support homology-directed repair at defined TJP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.