ZNF43 Double Nickase Plasmid (h): sc-416697-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZNF43 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZNF43 Double Nickase Plasmid (h) and ZNF43 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZNF43. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZNF43 Double Nickase Plasmid (h) | sc-416697-NIC | 20 µg | $410.00 | |||
ZNF43 Double Nickase Plasmid (h2) | sc-416697-NIC-2 | 20 µg | $410.00 |
ZNF43 encodes a zinc finger protein predicted to function in sequence-specific DNA binding and regulation of gene expression programs that shape cell state and lineage-specific transcription. As a putative nuclear factor, ZNF43 is relevant to pathways governing chromatin organization, transcriptional repression/activation dynamics, and maintenance of genomic stability through coordinated control of downstream target genes. Altered zinc finger protein networks are frequently associated with dysregulated differentiation and stress-response signaling, providing a rationale for interrogating ZNF43 in models of cell proliferation and transformation. Functional perturbation of ZNF43 supports mechanistic studies of transcriptional circuitry and genotype–phenotype relationships in human cells.
ZNF43 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZNF43 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZNF43. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZNF43 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZNF43-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.