Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Zic4 Double Nickase Plasmid (h): sc-407517-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Zic4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Zic4 Double Nickase Plasmid (h) and Zic4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZIC4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Zic4 Antibody (RY-6): sc-101202
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Zic4 Double Nickase Plasmid (h) | sc-407517-NIC | 20 µg | $410.00 | |||
Zic4 Double Nickase Plasmid (h2) | sc-407517-NIC-2 | 20 µg | $410.00 |
ZIC4 encodes the zinc finger transcription factor Zic4, a nuclear regulator that binds DNA to control gene expression programs during embryonic patterning and neurodevelopment. Zic4 participates in transcriptional networks that coordinate neuronal differentiation, regional identity in the developing brain, and cerebellar morphogenesis, intersecting with developmental signaling pathways such as WNT and SHH that shape dorsal midline and hindbrain structures. Altered ZIC4 dosage or disruption of its regulatory function has been associated with congenital brain malformations and neurodevelopmental phenotypes, making it a useful locus for studying transcriptional control in neural lineages. In cell-based models, ZIC4 perturbation helps define gene regulatory networks governing progenitor fate decisions, neuronal maturation, and lineage-specific chromatin states.
Zic4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZIC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZIC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZIC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZIC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.