ZAP-70 Antibody (A-8): sc-374347
- ZAP-70 Antibody (A-8) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 5-35 near the N-terminus of ZAP-70 of human origin
- recommended for detection of ZAP-70 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine
- See ZAP-70 (1E7.2): sc-32760 for ZAP-70 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ZAP-70 Antibody (A-8) for WB applications. These reagents are now offered in bundles with ZAP-70 Antibody (A-8) (see ordering information below).
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ZAP-70 Antibody (A-8) is a mouse monoclonal IgG1 antibody that detects ZAP-70 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ZAP-70, a crucial tyrosine kinase, plays a significant role in T cell receptor signaling, which is essential for T lymphocyte activation and subsequent immune responses. Located primarily in the cytoplasm, ZAP-70 associates with the ζ chain of the T cell receptor complex, where ZAP-70 becomes activated through phosphorylation in response to antigen engagement. This activation is vital for propagating downstream signaling pathways that lead to T cell proliferation and differentiation, making ZAP-70 a key player in adaptive immunity. The importance of ZAP-70 is underscored by ZAP-70′s involvement in various immunological disorders; mutations in the ZAP-70 gene can lead to severe combined immunodeficiency, highlighting ZAP-70′s critical function in maintaining a robust immune system. Anti-ZAP-70 antibody (A-8) is therefore an invaluable tool for researchers studying T cell signaling and its implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZAP-70 Antibody (A-8) References:
- ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia. | Crespo, M., et al. 2003. N Engl J Med. 348: 1764-75. PMID: 12724482
- ZAP-70 expression and prognosis in chronic lymphocytic leukaemia. | Orchard, JA., et al. 2004. Lancet. 363: 105-11. PMID: 14726163
- Intramolecular regulatory switch in ZAP-70: analogy with receptor tyrosine kinases. | Brdicka, T., et al. 2005. Mol Cell Biol. 25: 4924-33. PMID: 15923611
- ZAP70 in chronic lymphocytic leukaemia. | Amin, S., et al. 2008. Int J Biochem Cell Biol. 40: 1654-8. PMID: 17625948
- Zap-70 and CD38 as predictors of IgVH mutation in CLL. | Cruse, JM., et al. 2007. Exp Mol Pathol. 83: 459-61. PMID: 17931624
- Syk and Zap-70 function redundantly to promote angioblast migration. | Christie, TL., et al. 2010. Dev Biol. 340: 22-9. PMID: 20096681
- ZAP-70 expression is associated with increased risk of autoimmune cytopenias in CLL patients. | Zanotti, R., et al. 2010. Am J Hematol. 85: 494-8. PMID: 20575031
- Quantification of ZAP-70 mRNA by real-time PCR is a prognostic factor in chronic lymphocytic leukemia. | Wang, YH., et al. 2012. J Cancer Res Clin Oncol. 138: 1011-7. PMID: 22362302
- ZAP-70 expression and proliferative activity in chronic lymphocytic leukemia. | Šoljić, V., et al. 2013. Leuk Lymphoma. 54: 1171-6. PMID: 23098292
- Decoding the deactivation mechanism of R192W mutation of ZAP-70 using molecular dynamics simulations and binding free energy calculations. | Zhang, X., et al. 2023. J Mol Model. 29: 371. PMID: 37953318
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZAP-70 Antibody (A-8) | sc-374347 | 200 µg/ml | $322.00 | |||
ZAP-70 Antibody (A-8): m-IgG Fc BP-HRP Bundle | sc-540442 | 200 µg Ab; 10 µg BP | $361.00 | |||
ZAP-70 Antibody (A-8): m-IgG1 BP-HRP Bundle | sc-542125 | 200 µg Ab; 20 µg BP | $361.00 | |||
ZAP-70 (A-8) Neutralizing Peptide | sc-374347 P | 100 µg/0.5 ml | $69.00 |