Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
VPS39 Double Nickase Plasmid (h): sc-404507-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VPS39 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- VPS39 Double Nickase Plasmid (h) and VPS39 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting VPS39. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VPS39 Antibody (C-5): sc-514762
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VPS39 Double Nickase Plasmid (h) | sc-404507-NIC | 20 µg | $410.00 |
VPS39 encodes a core subunit of the homotypic fusion and vacuole protein sorting (HOPS) tethering complex that coordinates late endosome–lysosome and autophagosome–lysosome fusion events. Through interactions with Rab GTPases and SNARE machinery, VPS39 supports endolysosomal maturation, cargo delivery to lysosomes, and turnover of membrane proteins and organelles. Disruption of HOPS-dependent trafficking perturbs lysosomal homeostasis and autophagic flux, processes linked to neurodegeneration, infection biology, and tumor cell stress adaptation. As a result, VPS39 is widely studied in pathways controlling intracellular degradation, organelle dynamics, and signaling from endolysosomal compartments.
VPS39 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the VPS39 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within VPS39. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt VPS39 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of VPS39-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.