Vasohibin Double Nickase Plasmid (h): sc-403526-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Vasohibin-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Vasohibin-1 Double Nickase Plasmid (h) and Vasohibin-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting VASH1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Vasohibin-1 Antibody (C-6): sc-365541
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Vasohibin Double Nickase Plasmid (h) | sc-403526-NIC | 20 µg | $410.00 | |||
Vasohibin-1 Double Nickase Plasmid (h2) | sc-403526-NIC-2 | 20 µg | $410.00 |
VASH1 encodes vasohibin-1, an endothelial cell–associated regulator of angiogenesis that is induced by pro-angiogenic cues and functions in negative feedback control of vascular sprouting. Vasohibin modulates endothelial migration and proliferation and influences vessel maturation within signaling contexts that include VEGF-driven pathways and stress-responsive transcriptional programs. Altered VASH1 expression has been linked to changes in tumor vascularization, inflammatory microenvironments, and tissue remodeling, making it relevant for studies of neovascular biology and vascular homeostasis. In addition, VASH1 is studied in relation to cytoskeletal organization and endothelial barrier phenotypes that shape perfusion and permeability.
Vasohibin-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the VASH1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within VASH1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt VASH1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of VASH1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.