Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
USP14 Double Nickase Plasmid (h): sc-403167-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP14 Double Nickase Plasmid (h) and USP14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting USP14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USP14 Antibody (F-4): sc-398009
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP14 Double Nickase Plasmid (h) | sc-403167-NIC | 20 µg | $410.00 | |||
USP14 Double Nickase Plasmid (h2) | sc-403167-NIC-2 | 20 µg | $410.00 |
USP14 encodes a ubiquitin-specific protease that associates with the 26S proteasome and edits ubiquitin chains on substrates, thereby regulating proteasomal degradation and ubiquitin homeostasis in human cells. By trimming ubiquitin from conjugated proteins, USP14 influences protein quality control, cellular stress responses, and signaling pathways linked to proteostasis, including processes that impact cell-cycle progression and apoptosis. Altered USP14 activity has been studied in the context of dysregulated proteasome function and aberrant ubiquitin signaling, mechanisms frequently implicated in cancer biology and neurodegeneration. As a proteasome-associated deubiquitinase, USP14 is also relevant for investigating how ubiquitin dynamics shape antigen processing and broader immune-related protein turnover.
USP14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the USP14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within USP14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt USP14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of USP14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.