Anti-UBE2S Anticuerpo C-1 es un monoclonal de ratón IgG1 (kappa light chain) UBE2S Anticuerpo proporcionado como 200 µg/ml
producido contra los amino ácidos 1-87 localizados en el N-terminus de UBE2S de origen human
UBE2S Anticuerpo (C-1) es recomendado para detectar UBE2S de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA; también reactivo con otras especies, incluyendo anyd equine, canine and bovine
UBE2S Anticuerpo (C-1) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.
Contacte con nosotros para recibir GRATIS 10 µg de muestra de UBE2S (C-1): sc-390917.
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UBE2S Antibody (C-1) is a high quality monoclonal UBE2S antibody (also designated UBE2S antibody) suitable for the detection of the UBE2S protein of mouse, rat and human origin. UBE2S Antibody (C-1) is available as both the non-conjugated anti-UBE2S antibody form, as well as multiple conjugated forms of anti-UBE2S antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). UBE2S (ubiquitin-conjugating enzyme E2S), also known as EPF5 or E2EPF, is a 222 amino acid protein that belongs to the E2 family of ubiquitin-conjugating enzymes. Involved in the protein degradation pathway, UBE2S catalyzes the ATP-dependent attachment of ubiquitin (Ub) to target proteins, thereby tagging them for subsequent destruction by the proteasome. UBE2S is thought to increase the rate of tumor cell proliferation, invasion and metastasis through the VHL (von Hippel-Lindau) pathway, suggesting a role for UBE2S in carcinogenesis.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can UBE2S (C-1): sc-390917 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Preguntado por: Dr Ninau Qelp
Thank you for your question. Yes, UBE2S (C-1): sc-390917 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
Rated 5 de
Great Antibody for Western blotWorked well at a 1:200 dilution for a western blot using whole cell lysate from breast cancer cell lines.
Fecha de publicación: 2017-11-17
Rated 5 de
Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Fecha de publicación: 2015-01-05
Rated 5 de
Nice Western Blot data of UBE2S expression in Y79Nice Western Blot data of UBE2S expression in Y79, HeLa, MCF7 and PC-3 whole cell lysates. -SCBT QC
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