Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UBC13 Double Nickase Plasmid (h): sc-417204-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBC13 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- UBC13 Double Nickase Plasmid (h) and UBC13 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting UBE2N. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UBC13 Antibody (F-10): sc-376470
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBC13 Double Nickase Plasmid (h) | sc-417204-NIC | 20 µg | $410.00 | |||
UBC13 Double Nickase Plasmid (h2) | sc-417204-NIC-2 | 20 µg | $410.00 |
UBE2N encodes the E2 ubiquitin-conjugating enzyme UBC13, which forms a functional heterodimer with UBE2V1/UBE2V2 to catalyze K63-linked polyubiquitin chain assembly. These non-proteolytic ubiquitin signals coordinate DNA damage responses, innate immune and inflammatory signaling, and proteostasis, notably influencing NF-κB activation downstream of receptors such as TNFR and TLR/IL-1R through ubiquitination of pathway adaptors. UBC13-dependent ubiquitination also contributes to genome maintenance via ubiquitin signaling at sites of DNA lesions. Dysregulated UBE2N/UBC13 activity has been implicated in altered immune signaling and genomic instability phenotypes that are relevant to studies of inflammatory disorders and cancer biology.
UBC13 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the UBE2N locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within UBE2N. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt UBE2N function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of UBE2N-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.